Key Laboratory of MOE, Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai, China.
PLoS One. 2010 Dec 8;5(12):e15209. doi: 10.1371/journal.pone.0015209.
Ketamine is a widely used dissociative anesthetic which can induce some psychotic-like symptoms and memory deficits in some patients during the post-operative period. To understand its effects on neural population dynamics in the brain, we employed large-scale in vivo ensemble recording techniques to monitor the activity patterns of simultaneously recorded hippocampal CA1 pyramidal cells and various interneurons during several conscious and unconscious states such as awake rest, running, slow wave sleep, and ketamine-induced anesthesia. Our analyses reveal that ketamine induces distinct oscillatory dynamics not only in pyramidal cells but also in at least seven different types of CA1 interneurons including putative basket cells, chandelier cells, bistratified cells, and O-LM cells. These emergent unique oscillatory dynamics may very well reflect the intrinsic temporal relationships within the CA1 circuit. It is conceivable that systematic characterization of network dynamics may eventually lead to better understanding of how ketamine induces unconsciousness and consequently alters the conscious mind.
氯胺酮是一种广泛使用的分离麻醉剂，在术后期间，它可在一些患者中引起类似精神病的症状和记忆缺陷。为了了解它对大脑神经群体动力学的影响，我们采用了大规模的体内集合记录技术，以监测在几种意识和无意识状态（如清醒休息、跑步、慢波睡眠和氯胺酮诱导的麻醉）期间同时记录的海马 CA1 锥体神经元和各种中间神经元的活动模式。我们的分析表明，氯胺酮不仅在锥体细胞中，而且在至少七种不同类型的 CA1 中间神经元中诱导出明显的振荡动力学，包括假定的篮状细胞、钟摆细胞、双分层细胞和 O-LM 细胞。这些新出现的独特振荡动力学很可能反映了 CA1 回路中的内在时间关系。可以想象，对网络动力学的系统特征描述最终可能会导致更好地理解氯胺酮如何引起无意识，并因此改变意识。
