State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2740-2744. doi: 10.1099/ijs.0.028266-0. Epub 2010 Dec 24.
A novel actinomycete, strain FXJ7.203(T), was isolated from a coastal sediment sample collected in Bohai Bay, China. In 16S rRNA gene sequence analyses, strain FXJ7.203(T) always formed a unique monophyletic line within the genus Nonomuraea and was most closely related to Nonomuraea turkmeniaca (97.9 %), Nonomuraea candida (97.8 %), Nonomuraea helvata (97.7 %) and Nonomuraea rubra (97.5 %). Morphological and chemotaxonomic characteristics supported the allocation of the strain to the genus Nonomuraea. The polar lipid profile showed that its phospholipid type was PIV. The major fatty acids were iso-C(16 : 0), C(17 : 1)ω6c, iso-C(16 : 1) G, 10-methyl C(17 : 0) and iso-C(15 : 0). The major menaquinone was MK-9(H(4)) with minor amounts of MK-9(H(2)), MK-9(H(0)) and MK-9(H(6)). The results of physiological and biochemical tests allowed differentiation of strain FXJ7.203(T) from closely related species. Based on the genotypic and phenotypic data, strain FXJ7.203(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea maritima sp. nov. is proposed, with strain FXJ7.203(T) ( = CGMCC 4.5681(T) = NBRC 106687(T)) as the type strain.
一株新的放线菌，菌株 FXJ7.203(T)，从中国渤海湾的一个海岸沉积物样本中分离出来。在 16S rRNA 基因序列分析中，菌株 FXJ7.203(T)总是在游动放线菌属内形成一个独特的单系，与游动放线菌属的 Turkmenica（97.9%）、 Candida（97.8%）、 Helvata（97.7%）和 Rubra（97.5%）最为密切相关。形态学和化学分类学特征支持将该菌株分配到游动放线菌属。极性脂质谱表明其磷脂类型为 PIV。主要脂肪酸为 iso-C(16:0)、C(17:1)ω6c、iso-C(16:1)G、10-甲基 C(17:0)和 iso-C(15:0)。主要的menaquinone 是 MK-9(H(4))，少量的 MK-9(H(2))、MK-9(H(0))和 MK-9(H(6))。生理生化试验的结果允许将菌株 FXJ7.203(T)与密切相关的种区分开来。基于基因型和表型数据，菌株 FXJ7.203(T)代表游动放线菌属的一个新种，命名为 Nonomuraea maritima sp. nov.，其模式菌株为 FXJ7.203(T)（=CGMCC 4.5681(T)=NBRC 106687(T)）。
