College of Land Resources and Environment, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1409-1413. doi: 10.1099/ijs.0.034520-0. Epub 2011 Aug 5.
An actinomycete, designated FXJ1.102(T), was isolated from acidic soil collected in Jiangxi Province, south-east China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FXJ1.102(T) belonged to the genus Nonomuraea and was most closely related to Nonomuraea candida HMC10(T), Nonomuraea turkmeniaca DSM 43926(T), Nonomuraea antimicrobica YIM 61105(T) and 'Nonomuraea aegyptia' S136 (98.9, 98.3, 97.9 and 97.5 % 16S rRNA gene sequence similarities, respectively). The morphological characteristics were typical of the genus Nonomuraea. The chemotaxonomic properties, such as cell-wall chemotype IIIB, phospholipid type IV, MK-9(H(4)) as the major menaquinone and iso-C(16:0) (22.2 %) as the major fatty acid, supported the assignment of the strain to the genus Nonomuraea. DNA-DNA relatedness and physiological tests allowed genotypic and phenotypic differentiation of strain FXJ1.102(T) from its closest phylogenetic relatives. The isolate therefore represents a novel species, for which the name Nonomuraea jiangxiensis sp. nov. is proposed. The type strain is FXJ1.102(T) ( = CGMCC 4.6533(T) = NBRC 106679(T)).
一株放线菌，命名为 FXJ1.102(T)，从中国东南部江西省采集的酸性土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 FXJ1.102(T)属于诺卡氏菌属，与粘球菌属的诺卡氏菌 Nonomuraea candida HMC10(T)、土库曼诺卡氏菌 Nonomuraea turkmeniaca DSM 43926(T)、抗微生物诺卡氏菌 Nonomuraea antimicrobica YIM 61105(T)和‘埃及诺卡氏菌’S136 最为密切相关（分别为 98.9%、98.3%、97.9%和 97.5%的 16S rRNA 基因序列相似性）。形态学特征典型的属于诺卡氏菌属。化学分类学特性，如细胞壁化学型 IIIB、磷脂型 IV、主要的menaquinone 为 MK-9(H(4))和主要脂肪酸为 iso-C(16:0)（22.2%），支持该菌株被归为诺卡氏菌属。DNA-DNA 同源性和生理试验允许 FXJ1.102(T) 菌株与最接近的系统发育亲缘关系进行基因型和表型分化。因此，该分离物代表了一个新的物种，提议命名为江西诺卡氏菌（Nonomuraea jiangxiensis）sp. nov.。其模式菌株为 FXJ1.102(T)（=CGMCC 4.6533(T)=NBRC 106679(T)）。
