Research Center for Agrotechnology and Biotechnology, Toyohashi University of Technology, Toyohashi, Japan.
FEMS Microbiol Lett. 2011 Apr;317(2):138-42. doi: 10.1111/j.1574-6968.2011.02224.x. Epub 2011 Feb 7.
Two strains of aerobic acidophilic chemoorganotrophic bacteria designed strains AP8(T) and AP9 were isolated from acid mine drainage and acidic soil, respectively. These isolates were gram-negative, nonmotile cocci and coccobacilli measuring 0.5-0.8 μm in diameter. Cells were capsulated. Colonies on solid media were pink colored. The pH range for growth was 3.0-6.0 (optimum pH 4.5). Sugars, gluconate, and some amino acids were good carbon and energy sources for growth. The main components of cellular fatty acids were C(15:0) iso and C(16:1) ω7c. Menaquinone-8 was the major quinone. The G+C content of genomic DNA was 59.5%. Both strains had identical sequences of 16S rRNA genes that were most closely related to that of the type strain of Acidobacterium capsulatum (96% similarity). There were major differences between the isolates and A. capsulatum in cell morphology, carbon nutrition, and fatty acid profiles. Based on these phylogenetic and phenotypic data, we propose the name Acidipila rosea gen. nov., sp. nov. to accommodate the novel isolates. The type strain is AP8(T) (NBRC 107607(T), KCTC 23427(T)).
两株需氧嗜酸化能有机异养细菌，设计菌株 AP8(T)和 AP9，分别从酸性矿山排水和酸性土壤中分离得到。这些分离株为革兰氏阴性、非运动性球菌和球杆菌，直径为 0.5-0.8μm。细胞呈胶囊状。固体培养基上的菌落呈粉红色。生长的 pH 范围为 3.0-6.0（最佳 pH 4.5）。糖、葡萄糖酸盐和一些氨基酸是生长的良好碳源和能源。细胞脂肪酸的主要成分是 C(15:0)iso 和 C(16:1)ω7c。menaquinone-8 是主要的醌。基因组 DNA 的 G+C 含量为 59.5%。两株菌的 16S rRNA 基因序列完全相同，与嗜酸菌（ Acidobacterium capsulatum）的模式株最为密切相关（相似度为 96%）。分离株与嗜酸菌在细胞形态、碳营养和脂肪酸谱方面存在较大差异。基于这些系统发育和表型数据，我们建议将新型分离株命名为 Acidipila rosea gen. nov.，sp. nov.。模式株为 AP8(T)（NBRC 107607(T)，KCTC 23427(T)）。
