Benz W C, Siegel P J, Baer J
J Virol. 1978 Sep;27(3):475-82. doi: 10.1128/JVI.27.3.475-482.1978.
Low concentrations of adenine arabinoside inhibited growth of two Epstein-Barr virus producer cell lines in culture, while not significantly affecting a nonproducer cell line and a B-cell-negative line. These observations were extended to include freshly infected cells. Mitogen-stimulated human umbilical cord blood lymphocytes were unaffected by the drug at concentration levels that inhibited [3H]thymidine incorporation into the DNA of Epstein-Barr virus-stimulated cells. DNA synthesis in Epstein-Barr virus-superinfected Raji cells was also adversely affected by adenine arabinoside. However, these same low concentrations of adenine arabinoside in the triphosphate form produced less effect on DNA synthesis in nuclear systems and DNA polymerase assays than on growth or DNA synthesis in whole cells. Therefore the effects reported here of low concentrations of the drug on whole cells may be only in part related to DNA polymerase inhibition. The work reported here suggests that adenine arabinoside has multiple sites of action in infected cells.
低浓度的阿糖腺苷抑制了两种培养的爱泼斯坦-巴尔病毒产生细胞系的生长，而对非产生细胞系和B细胞阴性系没有显著影响。这些观察结果扩展到包括新感染的细胞。在抑制[3H]胸苷掺入爱泼斯坦-巴尔病毒刺激细胞DNA的浓度水平下，丝裂原刺激的人脐带血淋巴细胞不受该药物影响。阿糖腺苷也对爱泼斯坦-巴尔病毒超感染的拉吉细胞中的DNA合成产生不利影响。然而，这些相同低浓度的三磷酸阿糖腺苷对核系统中的DNA合成和DNA聚合酶测定的影响比对全细胞生长或DNA合成的影响小。因此，这里报道的该药物低浓度对全细胞的影响可能仅部分与DNA聚合酶抑制有关。这里报道的工作表明阿糖腺苷在感染细胞中有多个作用位点。
