超感染细胞中新合成的爱泼斯坦-巴尔病毒DNA的沉降特性。
Sedimentation characteristics of newly synthesized Epstein-Barr viral DNA in superinfected cells.
作者信息
Siegel P J, Clough W, Strominger J L
出版信息
J Virol. 1981 Jun;38(3):880-5. doi: 10.1128/JVI.38.3.880-885.1981.
Abstract
Replicating Epstein-Barr virus (EBV) DNA molecules isolated from superinfected Raji cells were shown to consist of 80S to 65S and 58S (mature) molecules Pulse-chase experiments showed that radioactive label of DNAS molecules with the larger sedimentation coefficients was partially chased into 58S labeled forms. Formation of large concatemers of viral DNA could not be detected at any time after superinfection. The continuous presence of the 65S viral DNA intermediate throughout the replicative cycle combined with the observed inhibition of EBV DNA synthesis by addition of nontoxic levels of ethidium bromide to the superinfected cell culture led us to propose that EBV replication proceeds via a relaxed circular DNA intermediate.
摘要
从超感染的拉吉细胞中分离出的复制型爱泼斯坦-巴尔病毒（EBV）DNA分子显示由80S至65S和58S（成熟）分子组成。脉冲追踪实验表明，沉降系数较大的DNA分子的放射性标记部分被追踪到58S标记形式。超感染后任何时候都未检测到病毒DNA大串联体的形成。在整个复制周期中65S病毒DNA中间体持续存在，再加上向超感染细胞培养物中添加无毒水平的溴化乙锭可观察到对EBV DNA合成的抑制，这使我们提出EBV复制通过松弛环状DNA中间体进行。
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