使用电化学阻抗谱法无标记检测 HeLa 细胞中端粒酶活性。
Label-free detection of telomerase activity in HeLa cells using electrochemical impedance spectroscopy.
机构信息
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, 350002, China.
出版信息
Chem Commun (Camb). 2011 Mar 21;47(11):3129-31. doi: 10.1039/c0cc05230b. Epub 2011 Jan 25.
PMID:21267487
Abstract
A simple assay based on electrochemical impedance spectroscopy (EIS) for detection of telomerase activity is developed, and it is demonstrated that the label-free EIS method is capable of detecting the telomerase activity in HeLa cells with a detection limit of 1000 HeLa cells without using any amplification technique.
摘要
开发了一种基于电化学阻抗谱（EIS）的简单分析物检测端粒酶活性的方法，证明无标记的 EIS 方法能够在不使用任何扩增技术的情况下，以 1000 个 HeLa 细胞的检测限检测 HeLa 细胞中的端粒酶活性。
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