Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua Dongjie, Hepingli, Beijing 100029, China.
Evid Based Complement Alternat Med. 2011;2011:613737. doi: 10.1155/2011/613737. Epub 2011 Jan 17.
Diabetic nephropathy is one of the most significant microvascular complications in patients with type 2 diabetics. The concise mechanism of diabetic nephropathy is unknown and there is no successful treatment. The objective of study was to investigate effects of Chinese herbs (Tangshen Formula) on diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. OLETF rats and LETO rats were divided into four groups: LETO control, OLETF diabetics, OLETF diabetics treated with Tangshen Formula, and OLETF diabetics treated with Monopril. Body weight, blood glucose, and 24 h urinary proteins were measured once every four weeks. Blood samples and kidney tissues were obtained for analyses of total cholesterol, triglyceride, whole blood viscosity, plasma viscosity, and pathohistological examination at 36 and 56 weeksrespectively. Untreated OLETF rats displayed diabetic nephropathy over the study period. Treatment of OLETF rats with Tangshen Formula attenuated the increases in blood glucose, body weight, 24 h urinary protein content, serum total cholesterol, whole blood viscosity and plasma viscosity at certain time. Treatment with Tangshen Formula also reduced glomerulosclerotic index and interstitial fibrotic index seen in OLETF rats. In conclusion, Tangshen Formula could attenuate the development of diabetic nephropathy in OLETF rat diabetic model.
糖尿病肾病是 2 型糖尿病患者最主要的微血管并发症之一。糖尿病肾病的确切发病机制尚不清楚，也没有成功的治疗方法。本研究旨在探讨中药（糖肾方）对 Otsuka Long-Evans Tokushima Fatty（OLETF）大鼠糖尿病肾病的作用。将 OLETF 大鼠和 LETO 大鼠分为四组：LETO 对照组、OLETF 糖尿病组、OLETF 糖尿病糖肾方治疗组和 OLETF 糖尿病 Monopril 治疗组。每四周测量一次体重、血糖和 24 小时尿蛋白。分别在 36 周和 56 周时采集血液样本和肾脏组织，用于分析总胆固醇、甘油三酯、全血黏度、血浆黏度和病理组织学检查。未治疗的 OLETF 大鼠在整个研究期间均出现糖尿病肾病。在一定时间内，糖肾方治疗 OLETF 大鼠可降低血糖、体重、24 小时尿蛋白含量、血清总胆固醇、全血黏度和血浆黏度的升高。糖肾方还可降低 OLETF 大鼠肾小球硬化指数和间质纤维化指数。综上所述，糖肾方可减轻 OLETF 大鼠糖尿病模型中糖尿病肾病的发生发展。
