Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139, USA.
J Biomed Opt. 2011 Jan-Feb;16(1):011005. doi: 10.1117/1.3522506.
Tomographic phase microscopy measures the 3-D refractive index distribution of cells and tissues by combining the information from a series of angle-dependent interferometric phase images. In the original device, the frame rate was limited to 0.1 frames per second (fps) by the technique used to acquire phase images, preventing measurements of moving or rapidly changing samples. We describe an improved tomographic phase microscope in which phase images are acquired via a spatial fringe pattern demodulation method, enabling a full tomogram acquisition rate of 30 fps. In addition, in this system the refractive index is calculated by a diffraction tomography algorithm that accounts for the effects of diffraction in the 3-D reconstruction. We use the instrument to quantitatively monitor rapid changes in refractive index within defined subregions of cells due to exposure to acetic acid or changes in medium osmolarity.
层析相位显微镜通过结合一系列角度相关的干涉相位图像的信息，来测量细胞和组织的三维折射率分布。在原始设备中，相位图像的采集技术将帧率限制在每秒 0.1 帧（fps），这使得无法对移动或快速变化的样本进行测量。我们描述了一种改进的层析相位显微镜，其中相位图像通过空间条纹图案解调方法获取，从而实现了 30 fps 的全层析图采集速率。此外，在这个系统中，折射率是通过一个考虑三维重建中衍射效应的衍射层析算法计算的。我们使用该仪器定量监测由于暴露于乙酸或介质渗透压变化而导致的细胞内特定亚区的折射率的快速变化。
