Pavliak V, Kogan G, Sláviková E, Sandula J, Masler L
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
J Basic Microbiol. 1990;30(8):587-95. doi: 10.1002/jobm.3620300816.
The immunochemical properties and structural features of D-mannans from two Candida parapsilosis strains were studied. Weak cross-reactivity of D-mannans with antisera produced to C. parapsilosis strains was observed, as well as significant differences in the mannans structures were found by means of methylation analysis, acetolysis and 1H-NMR spectroscopy. In view of the discrepancies found, new taxonomic identification of one strain was carried out. On the basis of assimilation of potassium nitrate and formation of hat-shaped spores as well as other differences found by investigation of physiological characteristics, one strain of C. parapsilosis was reidentified as Hansenula anomala. This work demonstrates that immunochemical and structural investigations of cell-wall polysaccharide components can serve as a basis for taxonomic identification of yeast strains.
研究了两株近平滑念珠菌菌株中D-甘露聚糖的免疫化学性质和结构特征。观察到D-甘露聚糖与针对近平滑念珠菌菌株产生的抗血清之间的弱交叉反应，并且通过甲基化分析、乙酰解和1H-NMR光谱法发现甘露聚糖结构存在显著差异。鉴于所发现的差异，对其中一个菌株进行了新的分类鉴定。基于对硝酸钾的同化作用、帽形孢子的形成以及通过生理特征研究发现的其他差异，将一株近平滑念珠菌重新鉴定为异常汉逊酵母。这项工作表明，细胞壁多糖成分的免疫化学和结构研究可作为酵母菌株分类鉴定的基础。
