Emory University School of Medicine, 1670 Clairmont Road, Mail Code 151, Decatur, GA 30033, USA.
J Clin Lipidol. 2007 Dec;1(6):593-8. doi: 10.1016/j.jacl.2007.10.008. Epub 2007 Oct 22.
Increased concentrations of reactive oxygen molecules are believed to be a driving force in inflammation. Although evident in tissue culture and animal models, it has been difficult to link reactive oxygen species (ROS) and inflammatory markers in humans. In patients recruited to represent a broad spectrum of risk factors, we investigated the relationship between the plasma concentration of oxygen radicals and high-sensitivity C-reactive protein (hs-CRP), utilizing a new chemistry with an easily oxidized chromophore.
ROS and hs-CRP were measured in blood from 59 fasting subjects selected to have variable risk predicted by classical risk factors. ROS were determined using the free oxygen radical monitor, which is an indirect colorimetric assay for the concentration of hydroperoxides in whole blood.
Using log transformation, the correlation between ROS and hs-CRP was r = 0.505 (P < 0.0001). This relationship between ROS and hs-CRP was comparable (r = 0.527, P = 0.001) in the subgroup not currently on statin therapy (n = 39). ROS were not correlated with Framingham risk, r = -0.027 (P = 0.84).
ROS directly measured in human blood correlates strongly with hs-CRP.
活性氧分子浓度的增加被认为是炎症的驱动力。尽管在组织培养和动物模型中已经很明显，但很难将活性氧物种（ROS）与人类的炎症标志物联系起来。在招募的代表广泛风险因素的患者中，我们利用一种具有易氧化生色团的新化学物质，研究了血浆中氧自由基与高敏 C 反应蛋白（hs-CRP）之间的关系。
利用经典风险因素预测的可变风险，从 59 名空腹受试者的血液中测量 ROS 和 hs-CRP。ROS 是使用游离氧自由基监测仪测定的，这是一种用于测定全血中过氧化物浓度的间接比色法。
使用对数转换，ROS 与 hs-CRP 之间的相关性为 r = 0.505（P < 0.0001）。在未接受他汀类药物治疗的亚组（n = 39）中，ROS 与 hs-CRP 之间的关系相当（r = 0.527，P = 0.001）。ROS 与 Framingham 风险无关，r = -0.027（P = 0.84）。
直接在人血液中测量的 ROS 与 hs-CRP 密切相关。
