Reeve Wayne, Chain Patrick, O'Hara Graham, Ardley Julie, Nandesena Kemanthi, Bräu Lambert, Tiwari Ravi, Malfatti Stephanie, Kiss Hajnalka, Lapidus Alla, Copeland Alex, Nolan Matt, Land Miriam, Hauser Loren, Chang Yun-Juan, Ivanova Natalia, Mavromatis Konstantinos, Markowitz Victor, Kyrpides Nikos, Gollagher Margaret, Yates Ron, Dilworth Michael, Howieson John
Stand Genomic Sci. 2010 Jan 28;2(1):77-86. doi: 10.4056/sigs.43526.
Ensifer (Sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual Medicago (medic) species. Strain WSM419 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from a M. murex root nodule collected in Sardinia, Italy in 1981. WSM419 was manufactured commercially in Australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first report of a complete genome sequence for a microsymbiont of the group of annual medic species adapted to acid soils. We reveal that its genome size is 6,817,576 bp encoding 6,518 protein-coding genes and 81 RNA only encoding genes. The genome contains a chromosome of size 3,781,904 bp and 3 plasmids of size 1,570,951 bp, 1,245,408 bp and 219,313 bp. The smallest plasmid is a feature unique to this medic microsymbiont.
苜蓿中华根瘤菌是多种一年生苜蓿属（苜蓿）植物有效的固氮微共生体。菌株WSM419是一种需氧、具运动性、不形成芽孢、革兰氏阴性杆菌，于1981年从意大利撒丁岛采集的硬刺苜蓿根瘤中分离得到。由于其固氮、腐生能力和耐酸性等特性，WSM419在1985年至1993年期间在澳大利亚作为一年生苜蓿的接种剂进行商业化生产。在此，我们描述了该生物体的基本特征，以及完整的基因组序列和注释。这是关于适应酸性土壤的一年生苜蓿类群微共生体完整基因组序列的首次报道。我们发现其基因组大小为6,817,576 bp，编码6,518个蛋白质编码基因和81个仅编码RNA的基因。该基因组包含一条大小为3,781,904 bp的染色体和3个大小分别为1,570,951 bp、1,245,408 bp和219,313 bp的质粒。最小的质粒是这种苜蓿微共生体独有的特征。
