Reeve Wayne, O'Hara Graham, Chain Patrick, Ardley Julie, Bräu Lambert, Nandesena Kemanthi, Tiwari Ravi, Malfatti Stephanie, Kiss Hajnalka, Lapidus Alla, Copeland Alex, Nolan Matt, Land Miriam, Ivanova Natalia, Mavromatis Konstantinos, Markowitz Victor, Kyrpides Nikos, Melino Vanessa, Denton Matthew, Yates Ron, Howieson John
Stand Genomic Sci. 2010 Feb 28;2(1):66-76. doi: 10.4056/sigs.44642.
Rhizobium leguminosarum bv trifolii is the effective nitrogen fixing microsymbiont of a diverse range of annual and perennial Trifolium (clover) species. Strain WSM2304 is an aerobic, motile, non-spore forming, Gram-negative rod, isolated from Trifolium polymorphum in Uruguay in 1998. This microsymbiont predominated in the perennial grasslands of Glencoe Research Station, in Uruguay, to competitively nodulate its host, and fix atmospheric nitrogen. Here we describe the basic features of WSM2304, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a nitrogen fixing microsymbiont of a clover species from the American center of origin. We reveal that its genome size is 6,872,702 bp encoding 6,643 protein-coding genes and 62 RNA only encoding genes. This multipartite genome was found to contain 5 distinct replicons; a chromosome of size 4,537,948 bp and four circular plasmids of size 1,266,105 bp, 501,946 bp, 308,747 bp and 257,956 bp.
三叶草根瘤菌三叶草生物型是多种一年生和多年生三叶草（苜蓿）物种的有效固氮微共生体。菌株WSM2304是一种需氧、能运动、不形成芽孢、革兰氏阴性杆菌，于1998年从乌拉圭的多形三叶草中分离得到。这种微共生体在乌拉圭格伦科研究站的多年生草原中占主导地位，能竞争性地与宿主形成根瘤并固定大气中的氮。在此，我们描述了WSM2304的基本特征以及完整的基因组序列和注释。这是来自美洲起源中心的苜蓿物种固氮微共生体的首个完整基因组序列。我们发现其基因组大小为6,872,702 bp，编码6,643个蛋白质编码基因和62个仅编码RNA的基因。该多分体基因组包含5个不同的复制子；一个大小为4,537,948 bp的染色体和四个大小分别为1,266,105 bp、501,946 bp、308,747 bp和257,956 bp的环状质粒。
