Kato T, Iijima H, Ishihara K, Kaneko T, Hirai K, Naito Y, Okuda K
Tokyo Dental College.
Bull Tokyo Dent Coll. 1990 Nov;31(4):301-7.
To evaluate the efficacy of Listerine, a solution for washing the oral cavity consisting of essential oils (thymol, methanol, eukalyptol) and methyl salicylate, minimum concentrations inhibiting the growth of various microorganisms in the oral cavity and the bactericidal effects on bacteria in the saliva and dental plaque were evaluated in vitro. Listerine inhibited the growth of microorganisms over a very broad range. The minimum concentration inhibiting growth of Listerine was a 4 to 32 fold dilution, 2-4 times as potent as the solution after elimination of active ingredient components, in 38 of 54 bacterial strains, indicating the efficacy of the active ingredient in the inhibition of the growth of microorganisms. Bactericidal action of Listerine against from bacteria isolated from saliva and dental plaque from 5 healthy normal subjects was tested. Listerine exhibited a potent bactericidal effect on bacteria in saliva and dental plaque. Most of the bacteria died after a 30 second exposure to Listerine. According to these results, Listerine appears to be effective as a solution used for cleansing the oral cavity and dentures.
为评估含有精油（百里酚、甲醇、桉叶油素）和水杨酸甲酯的口腔清洗液李斯特林的功效，在体外评估了其抑制口腔内各种微生物生长的最低浓度以及对唾液和牙菌斑中细菌的杀菌作用。李斯特林在很宽的范围内抑制微生物生长。在54株细菌中的38株中，抑制李斯特林生长的最低浓度为稀释4至32倍，比去除活性成分后的溶液效力高2至4倍，表明活性成分在抑制微生物生长方面的功效。测试了李斯特林对5名健康正常受试者唾液和牙菌斑中分离出的细菌的杀菌作用。李斯特林对唾液和牙菌斑中的细菌表现出强大的杀菌作用。大多数细菌在接触李斯特林30秒后死亡。根据这些结果，李斯特林似乎作为一种用于清洁口腔和假牙的溶液是有效的。
