S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
Netherlands Institute of Ecology, NL3631 AC Nieuwersluis, The Netherlands.
Int J Syst Evol Microbiol. 2012 Jan;62(Pt 1):118-123. doi: 10.1099/ijs.0.025924-0. Epub 2011 Feb 18.
An aerobic, pink-pigmented, budding bacterium, designated strain S26(T), was isolated from an acidic Sphagnum peat bog of north-western Russia. Cells were non-motile and spherical, occurring singly, in pairs or in short chains, and were able to attach to surfaces by means of a holdfast material. Strain S26(T) was a moderately acidophilic, mesophilic organism capable of growth at pH 3.2-7.1 (optimum at pH 4.8-5.0) and at 4-33 °C (optimum at 20-26 °C). Most sugars, several organic acids and polyalcohols were the preferred growth substrates. The major fatty acids were C(16:0), C(18:1)ω9c and C(18:2)ω6c,12c. The major neutral lipids were n-C(31:9) hydrocarbon and squalene; the polar lipids were phosphatidylglycerol, phosphatidylcholine and components with an unknown structure. The DNA G+C content of strain S26(T) was 62.2 mol%. 16S rRNA gene sequence analysis showed that strain S26(T) is a member of the order Planctomycetales. Among taxonomically characterized representatives of this order, highest levels of 16S rRNA gene sequence similarity (95.1-95.2%) were observed with strains of the non-filamentous, peat-inhabiting planctomycete Singulisphaera acidiphila. Strain S26(T) could be differentiated from Singulisphaera acidiphila based on pigmentation, significant differences in substrate utilization patterns, greater tolerance of acidic conditions and the presence of C(16:1)ω9c. Based on the data presented, strain S26(T) is considered to represent a novel species of the genus Singulisphaera, for which the name Singulisphaera rosea sp. nov. is proposed; the type strain is S26(T) (=DSM 23044(T)=VKM B-2599(T)).
一株需氧、粉红色、出芽的细菌，被命名为 S26(T) 菌株，是从俄罗斯西北部的酸性泥炭沼泽中分离出来的。细胞是不动的、球形的，单个存在，成双存在或成短链存在，并且能够通过附着物质附着在表面上。S26(T) 菌株是一种中度嗜酸、嗜温的生物体，能够在 pH 值为 3.2-7.1（最佳 pH 值为 4.8-5.0）和 4-33°C（最佳温度为 20-26°C）下生长。大多数糖、几种有机酸和多元醇是首选的生长基质。主要脂肪酸为 C(16:0)、C(18:1)ω9c 和 C(18:2)ω6c,12c。主要中性脂质为 n-C(31:9)烃和角鲨烯；极性脂质为磷脂酰甘油、磷脂酰胆碱和具有未知结构的成分。S26(T) 菌株的 DNA G+C 含量为 62.2 mol%。16S rRNA 基因序列分析表明，S26(T) 菌株是螺旋体目（Planctomycetales）的成员。在该目中分类学上已鉴定的代表菌株中，与非丝状、泥炭栖息的螺旋体 Singulisphaera acidiphila 菌株的 16S rRNA 基因序列相似性最高（95.1-95.2%）。S26(T) 菌株与 Singulisphaera acidiphila 菌株的区别在于色素、底物利用模式的显著差异、对酸性条件的更大耐受性和存在 C(16:1)ω9c。基于所提供的数据，S26(T) 菌株被认为代表了 Singulisphaera 属的一个新种，提议使用新名称 Singulisphaera rosea sp. nov.；其模式菌株为 S26(T)（=DSM 23044(T)=VKM B-2599(T)）。
