Dobek Georgina L, Godbey W T
Department of Comparative Medicine, Tulane University, USA.
J Vis Exp. 2011 Feb 6(48):2535. doi: 10.3791/2535.
In this straightforward procedure, bladder tumors are established in female C57 mice through the use of catheterization, local cauterization, and subsequent cell adhesion. After their bladders are transurethrally catheterized and drained, animals are again catheterized to permit insertion of a platinum wire into bladders without damaging the urethra or bladder. The catheters are made of Teflon to serve as an insulator for the wire, which will conduct electrical current into the bladder to create a burn injury. An electrocautery unit is used to deliver 2.5W to the exposed end of the wire, burning away extracellular layers and providing attachment sites for carcinoma cells that are delivered in suspension to the bladder through a subsequent catheterization. Cells remain in the bladder for 90 minutes, after which the catheters are removed and the bladders allowed to drain naturally. The development of tumor is monitored via ultrasound. Specific attention is paid to the catheterization technique in the accompanying video.
在这个简单的操作过程中，通过导尿、局部烧灼和随后的细胞黏附，在雌性C57小鼠体内建立膀胱肿瘤模型。在经尿道对小鼠膀胱进行导尿并排空尿液后，再次进行导尿，以便在不损伤尿道或膀胱的情况下将铂丝插入膀胱。导管由聚四氟乙烯制成，作为导线的绝缘体，导线将电流传导至膀胱以造成烧伤损伤。使用电灼装置向导线的暴露端输送2.5W的功率，烧掉细胞外层，为通过后续导尿悬浮输送至膀胱的癌细胞提供附着位点。细胞在膀胱内保留90分钟，之后取出导管，让膀胱自然排空。通过超声监测肿瘤的发展。随附视频中特别关注了导尿技术。
