应用库尔特计数器评估 HIV 患者外周血单个核细胞的平均细胞体积以确定细胞内药物浓度。
Evaluation of the mean corpuscular volume of peripheral blood mononuclear cells of HIV patients by a coulter counter to determine intracellular drug concentrations.
机构信息
Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149 Turin, Italy.
出版信息
Antimicrob Agents Chemother. 2011 Jun;55(6):2976-8. doi: 10.1128/AAC.01236-10. Epub 2011 Mar 14.
Abstract
The mean corpuscular volume (MCV) of peripheral blood mononuclear cells (PBMCs) was determined by Coulter Counter, and data were used to calculate the intracellular drug concentrations. A total of 574 PBMC samples were collected from 190 patients. The MCV was 282.9 fl (minimum, 207.0; maximum, 354.6), with a standard deviation of 8.8%. Previous reports have often used a fixed value of 400 fl for the MCV, which may result in artificially low estimates of the intracellular concentrations of antivirals.
摘要
外周血单个核细胞（PBMC）的平均红细胞体积（MCV）通过库尔特计数器进行测定，并用于计算细胞内药物浓度。从 190 名患者中总共收集了 574 个 PBMC 样本。MCV 为 282.9fl（最小值，207.0；最大值，354.6），标准差为 8.8%。之前的报告通常使用固定的 MCV 值 400fl，这可能导致抗病毒药物细胞内浓度的人为低估。
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