Kumar A, Agarwal S P, Ahuja A, Ali J, Choudhry R, Baboota S
Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India.
Pharmazie. 2011 Feb;66(2):111-4.
The objective of the present study was to develop a nanocarrier based formulation of nadifloxacin and to investigate its in vitro antimicrobial effect against Propionibacterium acnes. Nanocarrier based microemulsion formulations were prepared by aqueous titration method, using oleic acid as oil phase, Tween-80 as surfactant and ethanol as co-surfactant in different ratios. This procedure yielded monodisperse microemulsions exhibiting a mean droplet size in the range of 95-560 nm. This range of particle size is good to treat follicle related disorders like acne vulgaris because the size of follicles is in the range of 50-100 microm. Furthermore the optimized formulations were characterized for surface morphology by transmission electron microscopy and refractive index. The permeation studies were carried out using rat skin mounted in Franz diffusion cells. Flux of the optimised formulation was 2.24 times that of control. The diameter of inhibition zone of the microemulsion was found good but smaller than that of a clindamycin disc because of the higher therapeutic efficacy of clindamycin against P. acnes. The results indicated that the developed microemulsion shows promising results against P. acnes bacteria and may be a good approach for acne treatment.
本研究的目的是开发一种基于纳米载体的那氟沙星制剂，并研究其对痤疮丙酸杆菌的体外抗菌作用。采用水相滴定法制备了基于纳米载体的微乳制剂，以油酸为油相，吐温-80为表面活性剂，乙醇为助表面活性剂，采用不同比例。该方法得到了单分散微乳液，其平均液滴尺寸在95-560nm范围内。这个粒径范围有利于治疗与毛囊相关的疾病，如寻常痤疮，因为毛囊的大小在50-100微米范围内。此外，通过透射电子显微镜和折射率对优化后的制剂进行了表面形态表征。使用安装在Franz扩散池中的大鼠皮肤进行渗透研究。优化制剂的通量是对照的2.24倍。发现微乳的抑菌圈直径良好，但由于克林霉素对痤疮丙酸杆菌的治疗效果更高，所以比克林霉素盘的抑菌圈直径小。结果表明，所开发的微乳对痤疮丙酸杆菌显示出有希望的结果，可能是治疗痤疮的一种好方法。
