The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100080, PR China.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):390-396. doi: 10.1099/ijs.0.025502-0. Epub 2011 Mar 25.
A novel, moderately halophilic, rod-shaped bacterium, designated strain YIM D17(T), was isolated from a sample of sediment from a salt mine in Yunnan, south-western China. The taxonomy of strain YIM D17(T) was investigated using a polyphasic approach. Strain YIM D17(T) was Gram-stain-negative, strictly aerobic and non-motile and formed pink colonies on marine agar. Optimal growth occurred at 37 °C, pH 7.5-8.0 and in the presence of 10-15 % (w/v) NaCl. The major menaquinone was MK-7. The polar lipid profile was composed predominantly of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, one phospholipid, one glycolipid and one aminolipid. Minor amounts of other lipids were also detectable. The predominant cellular fatty acids were iso-C(17 : 1)ω9c/10-methyl-C(16 : 0) (24.0 %), iso-C(15 : 0) (23.6 %) and C(16 : 1)ω7c/C(16 : 1)ω6c (13.8 %). The DNA G+C content was 43.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct clade with the genera Gracilimonas and Balneola (both in the phylum Bacteroidetes) and was related to the species Gracilimonas tropica, Balneola vulgaris and Balneola alkaliphila, with sequence similarities of 85.6 %, 83.0 % and 82.8 % to the respective type strains. On the basis of its phenotypic, chemotaxonomic and phylogenetic features, strain YIM D17(T) represents a novel species of a new genus, for which the name Fodinibius salinus gen. nov., sp. nov. is proposed. The type strain is YIM D17(T) ( = ACCC 10716(T) = DSM 21935(T)).
一株新型、中度嗜盐、杆状细菌，被命名为 YIM D17(T) 菌株，从中国西南部云南省盐矿的沉积物样本中分离得到。使用多相方法研究了菌株 YIM D17(T)的分类学。菌株 YIM D17(T)革兰氏阴性，严格需氧，不运动，在海洋琼脂上形成粉红色菌落。最佳生长条件为 37°C、pH7.5-8.0 和 10-15%（w/v）NaCl。主要的类异戊二烯醌是 MK-7。极性脂谱主要由双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、一种磷脂、一种糖脂和一种胺脂组成。还可以检测到少量其他脂质。主要的细胞脂肪酸是 iso-C(17:1)ω9c/10-甲基-C(16:0)（24.0%）、iso-C(15:0)（23.6%）和 C(16:1)ω7c/C(16:1)ω6c（13.8%）。DNA G+C 含量为 43.0 mol%。基于 16S rRNA 基因序列比较的系统发育分析表明，该分离株与 Gracilimonas 和 Balneola 属（均属于 Bacteroidetes 门）形成一个独特的分支，与 Gracilimonas tropica、Balneola vulgaris 和 Balneola alkaliphila 种最为相关，与各自的模式株的序列相似性分别为 85.6%、83.0%和 82.8%。基于其表型、化学分类和系统发育特征，菌株 YIM D17(T)代表一个新属的一个新型种，命名为 Fodinibius salinus 属 nov.，sp. nov.。模式株为 YIM D17(T)（=ACCC 10716(T)=DSM 21935(T)）。
