Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China.
Technology Research Center, HongyunHonghe Tobacco (Group), Kunming, Yunnan 650202, P. R. China.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):3989-3993. doi: 10.1099/ijs.0.052043-0. Epub 2013 May 24.
A facultatively anaerobic, Gram-staining-negative, pale red-pigmented, non-motile, rod-shaped, moderately halophilic bacterium, designated strain YIM J14(T), was isolated from a sediment sample from a salt mine in Yunnan, south-western China. Growth occurred at NaCl concentrations of between 2 % and 15 % (w/v) and optimally with 5-9 % NaCl. The optimum temperature and pH for growth of the strain were 28 °C and pH 7.5. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 1ω9c/10-methyl-C16 : 0. The polar lipid profile was composed predominantly of diphosphatidylglycerol, phosphatidylcholine and one unknown phospholipid. Minor amounts of other lipids were also detectable. The genomic DNA G+C content was 47.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain YIM J14(T) was related to Gracilimonas tropica in the phylum Bacteroidetes. The level of 16S rRNA gene sequence similarity between strain YIM J14(T) and Gracilimonas tropica CL-CB462(T) was 96.9 %. A DNA-DNA hybridization experiment between strain YIM J14(T) and Gracilimonas tropica indicated levels of relatedness of 28 %. Chemotaxonomic data supported the placement of strain YIM J14(T) in the genus Gracilimonas. DNA-DNA hybridization and biochemical and physiological characterization allowed strain YIM J14(T) to be differentiated from Gracilimonas tropica. It is therefore considered to represent a novel species of the genus Gracilimonas, for which the name Gracilimonas mengyeensis sp. nov. is proposed. The type strain YIM J14(T) ( = ACCC 10717(T) = DSM 21985(T)).
一株兼性厌氧、革兰氏染色阴性、浅红色、非运动、杆状、中度嗜盐的细菌，被命名为 YIM J14(T) 菌株，从中国西南部云南省盐矿的沉积物样本中分离得到。该菌在 2%至 15%（w/v）的 NaCl 浓度下生长，最适生长的 NaCl 浓度为 5-9%。该菌生长的最适温度和 pH 值分别为 28°C 和 pH 7.5。主要的细胞脂肪酸为 iso-C15:0、anteiso-C15:0 和 iso-C17:1ω9c/10-methyl-C16:0。极性脂谱主要由二磷脂酰甘油、磷脂酰胆碱和一种未知的磷脂组成。还可以检测到少量其他脂质。基因组 DNA G+C 含量为 47.2mol%。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 YIM J14(T)与厚壁菌门的 Gracilimonas tropica 密切相关。菌株 YIM J14(T)与 Gracilimonas tropica CL-CB462(T)的 16S rRNA 基因序列相似性为 96.9%。菌株 YIM J14(T)与 Gracilimonas tropica 的 DNA-DNA 杂交实验表明，两者的相关性为 28%。化学分类学数据支持将菌株 YIM J14(T)归入 Gracilimonas 属。DNA-DNA 杂交和生化生理特性鉴定表明，菌株 YIM J14(T)与 Gracilimonas tropica 不同。因此，它被认为是 Gracilimonas 属的一个新种，现命名为 Gracilimonas mengyeensis sp. nov.。模式菌株 YIM J14(T)（ = ACCC 10717(T) = DSM 21985(T)）。
