猪尾猕猴模型的优化：性交情况下局部杀微生物剂的安全性
Pigtailed macaque model refinement: topical microbicide safety in the presence of coitus.
作者信息
Sweeney Yvonne T Cosgrove, Angeles Ray O, Cummings Peter K, Smith April D, Patton Dorothy L
机构信息
Department of Obstetrics & Gynecology, University of Washington, Seattle, WA, USA.
出版信息
J Med Primatol. 2011 Oct;40(5):327-34. doi: 10.1111/j.1600-0684.2011.00478.x. Epub 2011 Mar 28.
BACKGROUND
Inclusion of sexual activity in the macaque model for topical microbicide safety evaluation would more closely mimic human use of topical microbicides and provide a more rigorous safety assessment.
METHODS
Initially, male-female partners were monitored in cohousing arrangements to determine whether macaques would copulate ad libitum. The logistics of performing vaginal examinations before and after coital visits were analyzed and optimized. Findings from cervicovaginal examinations conducted before and after sexual activity were compared.
RESULTS
Coital activity was reliably observed in the majority of cohousing sessions, representing all phases of the menstrual cycle. Female macaques were trained to be restrained while fully alert for pre-coital vaginal sampling. Post-coital examinations occur under general sedation. Post-coital examinations reveal alterations to tissues, microbiology, and pH compared with pre-coital visits.
CONCLUSIONS
This work clearly demonstrates that it is feasible to incorporate sexual activity in the macaque model for topical microbicide safety assessment.
背景
将性行为纳入猕猴局部杀菌剂安全性评估模型，将更接近人类对局部杀菌剂的使用情况，并提供更严格的安全性评估。
方法
最初，对同居安排中的雌雄猕猴伴侣进行监测，以确定猕猴是否会随意交配。分析并优化了性交前后进行阴道检查的流程。比较了性活动前后宫颈阴道检查的结果。
结果
在大多数同居期间均能可靠观察到性交活动，涵盖月经周期的所有阶段。雌性猕猴经过训练，在完全清醒的状态下接受性交前阴道采样时保持克制。性交后检查在全身麻醉下进行。与性交前检查相比，性交后检查显示组织、微生物学和pH值发生了变化。
结论
这项工作清楚地表明，将性行为纳入猕猴局部杀菌剂安全性评估模型是可行的。
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