Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, United States.
ACS Nano. 2011 May 24;5(5):3651-9. doi: 10.1021/nn1035262. Epub 2011 Apr 6.
Porous Si nanoparticles, prepared from electrochemically etched single crystal Si wafers, function as photosensitizers to generate (1)O(2) in ethanol and in aqueous media. The preparation conditions for the porous Si nanoparticles were optimized to maximize (1) the yield of material; (2) its quantum yield of (1)O(2) production; and (3) its in vitro degradation properties. The optimal formulation was determined to consist of nanoparticles 146 ± 7 nm in diameter, with nominal pore sizes of 12 ± 4 nm. The quantum yield for (1)O(2) production is 0.10 ± 0.02 in ethanol and 0.17 ± 0.01 in H(2)O. HeLa or NIH-3T3 cells treated with 100 μg/mL porous Si nanoparticles and exposed to 60 J/cm(2) white light (infrared filtered, 100 mW/cm(2) for 10 min) exhibit ∼45% cell death, while controls containing no nanoparticles show 10% or 25% cell death, respectively. The dark control experiment yields <10% cytotoxicity for either cell type.
多孔硅纳米粒子，由电化学刻蚀的单晶硅片制备而成，可用作敏化剂，在乙醇和水介质中生成（1）O（2）。优化了多孔硅纳米粒子的制备条件，以最大限度地提高（1）材料的产率；（2）其（1）O（2）生成量子产率；以及（3）其体外降解性能。最佳配方确定为由直径 146±7nm 的纳米粒子组成，标称孔径为 12±4nm。在乙醇中（1）O（2）生成的量子产率为 0.10±0.02，在 H（2）O 中为 0.17±0.01。用 100μg/mL 多孔硅纳米粒子处理 HeLa 或 NIH-3T3 细胞，并暴露于 60J/cm（2）白光（红外过滤，100mW/cm（2）照射 10 分钟），可导致约 45%的细胞死亡，而不含纳米粒子的对照细胞分别显示出 10%或 25%的细胞死亡。暗对照实验对这两种细胞类型的细胞毒性均<10%。
