Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, 14.801-903, Araraquara, SP, Brazil.
Lasers Med Sci. 2012 Mar;27(2):403-11. doi: 10.1007/s10103-011-0909-y. Epub 2011 Apr 6.
The aims of this study were to evaluate the effects of Photogem®-mediated photosensitization on rat palatal mucosa and the biodistribution of the photosensitizer in this tissue. A solution of Photogem® (500 or 1000 mg/l) was applied to the palatal mucosa for 30 min and the exposure time to blue LED (460 nm) was 20 min (144 J/cm(2)). At 0, 1, 3, and 7 days, palatal mucosa was photographed for macroscopic analysis. After killing, the palate was removed for microscopic analysis. Thermal mapping evaluated temperature change in the tissue during irradiation. All experimental groups revealed intact mucosa in the macroscopic analysis. Tissue alterations were observed microscopically for only four out of 80 animals subjected to PDT. Fluorescence emitted by Photogem® was identified and was limited to the epithelial layer. A temperature increase from 35 to 41°C was recorded. Photogem®- mediated PDT was not toxic to the rat palatal mucosa.
本研究的目的是评估 Photogem®介导的光动力疗法对大鼠腭黏膜的影响，以及该组织中光敏剂的分布。将 Photogem®（500 或 1000mg/L）溶液应用于腭黏膜 30 分钟，然后用 460nm 的蓝光 LED 照射 20 分钟（144J/cm²）。在 0、1、3 和 7 天时，对腭黏膜进行拍照进行宏观分析。处死动物后，取下腭进行显微镜分析。热图评估了辐照过程中组织温度的变化。所有实验组在宏观分析中均显示黏膜完整。仅在 80 只接受 PDT 的动物中有 4 只在显微镜下观察到组织改变。识别出 Photogem®发出的荧光，仅限于上皮层。记录到温度从 35°C 升高到 41°C。Photogem®介导的光动力疗法对大鼠腭黏膜没有毒性。
