Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Tr. SNP 1, 040 11, Košice, Slovak Republic.
In Vitro Cell Dev Biol Anim. 2011 Jun;47(5-6):376-82. doi: 10.1007/s11626-011-9405-8. Epub 2011 Apr 13.
Humic acids are known for their overall positive health and productivity effects in animal feeding trials and, controversially, as an aetiological factor of cancer. We tried to assess the in vitro effect of humic acids from a selected source in Slovakia when used at recommended prophylactic dosage. We investigated antioxidant properties, enzymatic and non-enzymatic antioxidant defence system in liver mitochondria and cultured cancer cell lines in vitro. We observed a significant decrease in superoxide dismutase activity after humic acids treatment irrespective of dissolving in dimethyl sulphoxide or direct addition to mitochondria suspension in a respiration medium. Activities of other antioxidant enzymes measured, such as glutathione peroxidase and glutathione reductase, showed no significant differences from the control as well as the reduced glutathione content. Percentage of inhibition by humic acids of superoxide radical indicated lower efficacy compared with that of hydroxyl radical. Survival of six different cancer cells lines indicated that only the acute T lymphoblastic leukaemia cell line was sensitive to the tested humic acids. Despite relatively low solubility in aqueous solutions, humic acids from the selected source participated in redox regulation. By recapturing the radicals, humic acids reloaded the antioxidant defensive mechanism. Results from in vitro study conducted with humic acids from the natural source showed potential of these substances as promising immunity enhancing agents.
腐殖酸在动物饲养试验中因其对整体健康和生产力的积极影响而闻名，但其也被认为是癌症的病因之一。我们试图评估来自斯洛伐克选定来源的腐殖酸在推荐预防剂量下的体外效应。我们研究了抗氧化特性、肝线粒体中的酶和非酶抗氧化防御系统以及体外培养的癌细胞系。我们观察到，在用二甲基亚砜溶解或直接添加到呼吸介质中的线粒体悬浮液中后，腐殖酸处理后超氧化物歧化酶活性显著降低。所测量的其他抗氧化酶，如谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性与对照以及还原型谷胱甘肽含量相比没有显著差异。与羟自由基相比，腐殖酸对超氧自由基的抑制百分率表明其功效较低。六种不同癌细胞系的存活率表明，只有急性 T 淋巴细胞白血病细胞系对测试的腐殖酸敏感。尽管在水溶液中的溶解度相对较低，但来自选定来源的腐殖酸参与了氧化还原调节。通过捕获自由基，腐殖酸重新加载了抗氧化防御机制。来自天然来源腐殖酸的体外研究结果表明，这些物质具有作为有前途的免疫增强剂的潜力。
