Division of Digestive Diseases, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
J Med Virol. 2011 Jul;83(7):1142-50. doi: 10.1002/jmv.22090. Epub 2011 Apr 22.
The high frequency of mutation during hepatitis B virus (HBV) infection has resulted in 8 genotypes (A-H) with varying effects on disease severity and treatment efficacy. However, analysis of intrapatient HBV diversity is limited, especially during HIV co-infection. Therefore, a preliminary study was performed to analyze HBV X gene diversity in 17 HBV/HIV co-infected individuals. Phylogenetic analysis revealed HBV genotype A in 13 individuals (76.5%) or genotype E in 1 individual (5.9%). Additionally, 3 individuals were dually infected with HBV genotypes A and G (17.6%). Overall, higher genetic distance and entropy were observed in the X region and overlapping polymerase (Pol(X)) regions when compared to the PreS, S, and overlapping polymerase (Pol(PS) and Pol(S)) regions analyzed in the same patients as part of a previous study. In addition, multiple viral variants from 2 individuals with dual HBV infection did not group with either genotype A or G by phylogenetic analysis, indicating possible recombination. SimPlot bootscan analysis confirmed recombination breakpoints within the X gene in both individuals. Recombination between HBV genotypes may represent an important evolutionary strategy that enhances overall pathogenic potential and/or alters the downstream effects of the HBV X protein.
乙型肝炎病毒 (HBV) 感染过程中的高频突变导致了 8 种基因型 (A-H)，这些基因型对疾病严重程度和治疗效果有不同的影响。然而，对患者体内 HBV 多样性的分析受到限制，特别是在 HIV 合并感染的情况下。因此，进行了一项初步研究，以分析 17 例 HBV/HIV 合并感染个体的 HBV X 基因多样性。系统进化分析显示，13 例（76.5%）为基因型 A，1 例（5.9%）为基因型 E。此外，还有 3 例同时感染了基因型 A 和 G（17.6%）。总体而言，与之前研究中分析的同一患者的 PreS、S 和重叠聚合酶（Pol(PS)和 Pol(S)）区域相比，X 区和重叠聚合酶（Pol(X)）区的遗传距离和熵更高。此外，来自 2 例双重 HBV 感染个体的多个病毒变异体在系统进化分析中没有与基因型 A 或 G 聚为一组，表明可能存在重组。SimPlot 引导扫描分析证实了这两个人的 X 基因内存在重组断点。HBV 基因型之间的重组可能代表一种重要的进化策略，增强了整体致病潜力和/或改变了 HBV X 蛋白的下游效应。
