Milei José, Otero Losada Matilde, Gómez Llambí Hernán, Grana Daniel R, Suárez Daniel, Azzato Francisco, Ambrosio Giuseppe
José Milei, Matilde Otero Losada, Hernán Gómez Llambí, Daniel R Grana, Daniel Suárez, Francisco Azzato, Instituto de Investigaciones Cardiológicas, University of Buenos Aires - CONICET, Buenos Aires, C1122AAJ, Argentina.
World J Cardiol. 2011 Apr 26;3(4):111-6. doi: 10.4330/wjc.v3.i4.111.
To investigate the effects of chronic drinking of cola beverages on metabolic and echocardiographic parameters in rats.
Forty-eight male Wistar rats were divided in 3 groups and allowed to drink regular cola (C), diet cola (L), or tap water (W) ad libitum during 6 mo. After this period, 50% of the animals in each group were euthanized. The remaining rats drank tap water ad libitum for an additional 6 mo and were then sacrificed. Rat weight, food, and beverage consumption were measured regularly. Biochemical, echocardiographic and systolic blood pressure data were obtained at baseline, and at 6 mo (treatment) and 12 mo (washout). A complete histopathology study was performed after sacrifice.
After 6 mo, C rats had increased body weight (+7%, P < 0.01), increased liquid consumption (+69%, P < 0.001), and decreased food intake (-31%, P < 0.001). C rats showed mild hyperglycemia and hypertriglyceridemia. Normoglycemia (+69%, P < 0.01) and sustained hypertriglyceridemia (+69%, P < 0.01) were observed in C after washout. Both cola beverages induced an increase in left ventricular diastolic diameter (C: +9%, L: +7%, P < 0.05 vs W) and volumes (diastolic C: +26%, L: +22%, P < 0.01 vs W; systolic C: +24%, L: +24%, P < 0.05 vs W) and reduction of relative posterior wall thickness (C: -8%, L: -10%, P < 0.05 vs W). Cardiac output tended to increase (C: +25%, P < 0.05 vs W; L: +17%, not significant vs W). Heart rate was not affected. Pathology findings were scarce, related to aging rather than treatment.
This experimental model may prove useful to investigate the consequences of high consumption of soft drinks.
研究长期饮用可乐饮料对大鼠代谢和超声心动图参数的影响。
将48只雄性Wistar大鼠分为3组，在6个月内随意饮用常规可乐（C组）、健怡可乐（L组）或自来水（W组）。在此期间结束后，每组50%的动物被安乐死。其余大鼠再随意饮用自来水6个月，然后处死。定期测量大鼠体重、食物和饮料摄入量。在基线、6个月（治疗期）和12个月（洗脱期）时获取生化、超声心动图和收缩压数据。处死大鼠后进行完整的组织病理学研究。
6个月后，C组大鼠体重增加（+7%，P<0.01），液体摄入量增加（+69%，P<0.001），食物摄入量减少（-31%，P<0.001）。C组大鼠出现轻度高血糖和高甘油三酯血症。洗脱期后，C组大鼠血糖正常（+69%，P<0.01），高甘油三酯血症持续存在（+69%，P<0.01）。两种可乐饮料均导致左心室舒张直径增加（C组：+9%，L组：+7%，与W组相比P<0.05）和容积增加（舒张期C组：+26%，L组：+22%，与W组相比P<0.01；收缩期C组：+24%，L组：+24%，与W组相比P<0.05），相对后壁厚度减小（C组：-8%，L组：-10%，与W组相比P<0.05）。心输出量有增加趋势（C组：+25%，与W组相比P<0.05；L组：+17%，与W组相比无统计学意义）。心率未受影响。病理结果较少，与衰老而非治疗有关。
该实验模型可能对研究软饮料高消费的影响有用。
