Hygiene and Medical Microbiology, Medical University Innsbruck, Fritz Pregl Straße 1-3/III, Innsbruck, Austria.
Eur J Clin Microbiol Infect Dis. 2012 Jan;31(1):77-81. doi: 10.1007/s10096-011-1279-x. Epub 2011 May 10.
In the last several years, West Nile virus (WNV) was proven to be present especially in the neighboring countries of Austria, such as Italy, Hungary, and the Czech Republic, as well as in eastern parts of Austria, where it was detected in migratory and domestic birds. In summer 2010, infections with WNV were reported from Romania and northern Greece with about 150 diseased and increasingly fatal cases. We tested the sera of 1,607 blood donors from North Tyrol (Austria) and South Tyrol (Italy) for antibodies against WNV by using IgG enzyme-linked immunosorbent assay (ELISA). Initial results of the ELISA tests showed seroprevalence rates of 46.2% in North Tyrol and 0.5% in South Tyrol, which turned out to be false-positive cross-reactions with antibodies against tick-borne encephalitis virus (TBEV) by adjacent neutralization assays. These results indicate that seropositivity against WNV requires confirmation by neutralization assays, as cross-reactivity with TBEV is frequent and because, currently, WNV is not endemic in the study area.
在过去的几年中，西尼罗河病毒（WNV）已被证明存在于奥地利的邻国，如意大利、匈牙利和捷克共和国，以及奥地利东部地区，在那里已在迁徙和家养鸟类中检测到该病毒。2010 年夏天，罗马尼亚和希腊北部报告了 WNV 感染病例，约有 150 例患病和日益致命的病例。我们通过 IgG 酶联免疫吸附试验（ELISA）检测了来自北蒂罗尔（奥地利）和南蒂罗尔（意大利）的 1607 名献血者的血清，以检测针对 WNV 的抗体。ELISA 试验的初步结果显示，北蒂罗尔的血清阳性率为 46.2%，而南蒂罗尔为 0.5%，通过相邻的中和试验证实这些结果是与 tick-borne encephalitis virus（TBEV）的抗体的假阳性交叉反应。这些结果表明，针对 WNV 的血清阳性需要通过中和试验来确认，因为与 TBEV 的交叉反应很常见，并且由于目前该病毒在研究区域并非地方性流行。
