由猪传染性胃肠炎病毒（TGEV）诺齐利弱毒株诱导产生的针对猪传染性胃肠炎的泌乳免疫：仔猪的被动保护以及通过酶联免疫吸附测定法检测血清和乳汁中的抗体类别
Lactogenic immunity to transmissible gastroenteritis (TGE) of swine induced by the attenuated Nouzilly strain of TGE virus: passive protection of piglets and detection of serum and milk antibody classes by ELISA.
作者信息
Bernard S, Shirai J, Lantier I, Bottreau E, Aynaud J M
机构信息
Institut National de la Recherche Agronomique, Laboratoire de Pathologie Porcine, Nouzilly, France.
出版信息
Vet Immunol Immunopathol. 1990 Jan;24(1):37-47. doi: 10.1016/0165-2427(90)90076-5.
Piglets of eight sows vaccinated by different routes with the attenuated TGE mutant coronavirus, Nouzilly (N) strain, and piglets from two field seropositive sows were challenged with a virulent TGE strain. On the day of challenge and 10 days after challenge, milk and serum samples from sows were analysed for their level of neutralizing antibodies, total immunoglobulin classes and TGE antibody classes by an ELISA. No direct relationship was seen between the level of protection of the litters and the titres of the different antibody classes on the day of challenge. However, an inverse correlation was seen 10 days after challenge between protection and the level of TGE antibodies.
用减毒的传染性胃肠炎（TGE）突变冠状病毒努济耶（N）株通过不同途径对8头母猪进行疫苗接种，并对2头田间血清阳性母猪所产仔猪用强毒TGE毒株进行攻毒。在攻毒当天和攻毒后10天，通过酶联免疫吸附测定法（ELISA）分析母猪的乳汁和血清样本，检测其中和抗体水平、总免疫球蛋白类别以及TGE抗体类别。在攻毒当天，仔猪的保护水平与不同抗体类别的滴度之间未发现直接关系。然而，在攻毒后10天，保护水平与TGE抗体水平之间呈负相关。
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