Center for Advanced Studies in Ecology and Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
Mol Ecol Resour. 2009 May;9(3):937-9. doi: 10.1111/j.1755-0998.2009.02523.x. Epub 2009 Jan 22.
A total of nine microsatellite loci were isolated and characterized in the Chilean kelp Lessonia nigrescens Bory. Using two different enriched libraries, we observed 1-14 alleles per locus in two samples of 21 kelp individuals each. The observed heterozygosities ranged from 0.05 to 0.80 and all loci are in Hardy-Weinberg equilibrium for one or both samples. Seventeen samples collected from different sites showed high allele diversity along the species distribution. The variation detected at these markers is currently being used for the study of populations of Lessonia nigrescens at different geographical scales.
共分离鉴定出智利巨藻 Lessonia nigrescens Bory 中的 9 个微卫星位点。使用两种不同的富集文库，在 21 个巨藻个体的两个样本中，每个位点观察到 1-14 个等位基因。观察到的杂合度范围从 0.05 到 0.80，所有位点在一个或两个样本中均处于哈迪-温伯格平衡。从不同地点收集的 17 个样本显示出沿物种分布的高等位基因多样性。目前正在利用这些标记物检测到的变异来研究不同地理尺度的 Lessonia nigrescens 种群。
