Nayak Aishwarya Gajanan, Fernandes Aquaviva, Kulkarni Raghavendra, Ajantha Ganavalli Subramanyam, Lekha Krishnapillai, Nadiger Ramesh
1 Shree Dharmastal Manjunatheshwar College of Dental Sciences and Hospital, Dharwad, India.
J Oral Implantol. 2014 Feb;40(1):11-4. doi: 10.1563/AAID-JOI-D-10-00167. Epub 2011 May 16.
Gaps and hollow spaces at the implant abutment interface will act as a bacterial reservoir that may cause peri-implantitis. Hence, the sealing ability of O-ring (in addition to polysiloxane) and GapSeal (an antibacterial sealing gel) was evaluated. A total of 45 identical implant systems (ADIN Dental Implant Systems) were divided into 3 groups of 15 implants each: an unsealed group, a group sealed with O-rings, and a group sealed with GapSeal gel. The implant and abutment were gamma sterilized after assembly. Two implants from each group were randomly incubated in sterile brain heart infusion (BHI) broth tubes and checked for sterility. The remaining 13 implants were incubated in BHI broth inoculated with Enterococcus and incubated for 5 days. They were then removed from the tubes, dried aseptically, placed in 2% sodium hypochlorite solution for 30 minutes, and washed with sterile saline for 5 minutes. Next, the assembly was dried aseptically and put in sterile BHI broth tubes and incubated for 24 hours to check surface sterility. Keeping 2 implants as controls from each group, the remaining 11 implants were dismantled group-wise and placed in liquid BHI broth; the test tubes were then shaken thoroughly so that the broth would come in contact with all implant surfaces. The solution from this tube was poured on pre-prepared sterile agar plates and incubated for 24 hours. The colonies formed on the agar plate were then counted using a digital colony counter. The data thus obtained were subjected to statistical analysis by Kruskal-Wallis analysis of variance and Mann-Whitney U test. It was concluded that though microbial growth is seen in all the 3 groups, the least growth was seen in the GapSeal group followed by the O-ring group.
种植体基台界面处的缝隙和中空空间会成为细菌储存库，可能导致种植体周围炎。因此，对O形环（除聚硅氧烷外）和GapSeal（一种抗菌密封凝胶）的密封能力进行了评估。总共45个相同的种植系统（ADIN牙科种植系统）被分为3组，每组15个种植体：未密封组、用O形环密封的组和用GapSeal凝胶密封的组。种植体和基台组装后进行伽马灭菌。每组随机选取2个种植体在无菌脑心浸液（BHI）肉汤管中培养，并检查无菌情况。其余13个种植体在接种肠球菌的BHI肉汤中培养5天。然后将它们从管中取出，无菌干燥，置于2%次氯酸钠溶液中30分钟，并用无菌生理盐水冲洗5分钟。接下来，将组件无菌干燥，放入无菌BHI肉汤管中培养24小时，以检查表面无菌情况。每组保留2个种植体作为对照，其余11个种植体按组拆卸，放入液体BHI肉汤中；然后将试管充分摇晃，使肉汤与所有种植体表面接触。将该试管中的溶液倒在预先准备好的无菌琼脂平板上，培养24小时。然后使用数字菌落计数器对琼脂平板上形成的菌落进行计数。通过Kruskal-Wallis方差分析和Mann-Whitney U检验对所得数据进行统计分析。得出的结论是，尽管在所有3组中都观察到了微生物生长，但GapSeal组的生长最少，其次是O形环组。
