Tumor Suppression Group, Spanish National Cancer Research Center, Madrid, Spain.
Cell Cycle. 2011 Jul 1;10(13):2215-7. doi: 10.4161/cc.10.13.16185.
Dietary restriction (DR) has multiple beneficial effects, the two most prominently studied being an increased longevity and an increased cancer protection. Mammalian Sirt1 is a protein deacetylase that has been linked to DR. To explore the relation between Sirt1 and DR, we have examined here DR-induced cancer protection in mice overexpressing Sirt1 (2-3 fold) under its own regulatory elements (Sirt1-tg mice). In particular, we have subjected p53‑deficient mice, carrying or not the Sirt1-tg allele, to every-other-day fasting (EOD), which is a type of DR that significantly delays cancer onset. As expected, EOD extended the survival of p53-heterozygous (p53 (+/-) ) mice. However, the extension of survival of p53-heterozygous mice by EOD was the same in the presence or absence of the Sirt1-tg allele. These results suggest that Sirt1 has a limited role in mediating cancer protection by DR in mammals.
饮食限制（DR）具有多种有益作用，其中两个最受关注的作用是延长寿命和增强抗癌能力。哺乳动物 Sirt1 是一种去乙酰化酶，与 DR 有关。为了探讨 Sirt1 与 DR 的关系，我们在此研究了在自身调节元件（Sirt1-tg 小鼠）下过表达 Sirt1（2-3 倍）的小鼠中 DR 诱导的抗癌保护作用。具体而言，我们使携带或不携带 Sirt1-tg 等位基因的 p53 缺陷型小鼠进行隔日禁食（EOD），这是一种可显著延迟癌症发生的 DR 类型。正如预期的那样，EOD 延长了 p53 杂合子（p53（ +/- ））小鼠的生存时间。然而，EOD 对 p53 杂合子小鼠的生存时间的延长在 Sirt1-tg 等位基因存在或不存在的情况下是相同的。这些结果表明，Sirt1 在介导哺乳动物 DR 的抗癌保护作用方面的作用有限。
