Pácová Z, Kocur M
Zentralbl Bakteriol Orig A. 1978 Oct;241(4):481-7.
1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. The microtest was devised to allow results to be read after 4 h cultivation. Phosphatase activity was found in wide range of species and strains. Besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: Bacillus cereus, B. licheniformis, Aeromonas spp., Vibrio parahaemolyticus, Actinobacillus spp., Pasteurella spp., Xanthomonas spp., Flavobacterium spp., Alteromonas putrefaciens, Pseudomonas maltophilia, Ps. cepacia, and some other species of Pseudomonas. The species which gave uniformly negative phosphatase reaction were as follows: Staph. saprophyticus, Acinetobacter calcoaceticus, Alcaligenes faecalis, and Bordetella bronchiseptica.
采用传统平板法和微量试验对1115株需氧菌和兼性厌氧菌进行磷酸酶活性检测。微量试验设计为在培养4小时后读取结果。在多种菌种和菌株中均发现了磷酸酶活性。除了成功应用磷酸酶检测的葡萄球菌外，该检测还可作为以下菌种鉴别的重要检测方法之一：蜡样芽孢杆菌、地衣芽孢杆菌、气单胞菌属、副溶血性弧菌、放线杆菌属、巴斯德菌属、黄单胞菌属、黄杆菌属、腐败希瓦氏菌、嗜麦芽窄食单胞菌、洋葱伯克霍尔德菌以及其他一些假单胞菌属菌种。磷酸酶反应均呈阴性的菌种如下：腐生葡萄球菌、醋酸钙不动杆菌、粪产碱杆菌和支气管败血波氏杆菌。
