杜邦疱疹病毒酶联免疫吸附测定法（Du Pont Herpchek HSV ELISA）的多中心临床评估，这是一种用于直接检测单纯疱疹病毒的新型快速诊断测试。

Multicenter clinical evaluation of the Du Pont Herpchek HSV ELISA, a new rapid diagnostic test for the direct detection of herpes simplex virus.

作者信息

Baker D A, Pavan-Langston D, Gonik B, Milch P O, Dunkel E C, Berkowitz A, Woodin M B, Philip A, Vander-Mallie R J, Bobrow M N

机构信息

Department of Obstetrics and Gynecology, State University of New York, Stony Brook 11794.

出版信息

Adv Exp Med Biol. 1990;263:71-6. doi: 10.1007/978-1-4613-0601-6_6.

DOI:10.1007/978-1-4613-0601-6_6

PMID:2162132

Abstract

A new 4h rapid enzyme-immunoassay for direct detection of herpes simplex virus (HSV) antigen (Du Pont Herpchek) was evaluated with 743 clinical samples collected at obstetrics and gynecology (OB/GYN), sexually transmitted diseases (STD), and ophthalmology clinics. The sensitivity and specificity of Herpchek was 98.0% and 98.4% respectively compared to virus isolation in cell culture. Confirmatory blocking ELISA tests, clinical history and follow up indicate that the true specificity of the test is 100%.

摘要

采用一种新型的4小时快速酶免疫分析法（杜邦疱疹检查法）对从妇产科、性传播疾病科和眼科诊所收集的743份临床样本进行单纯疱疹病毒（HSV）抗原直接检测。与细胞培养中的病毒分离法相比，疱疹检查法的灵敏度和特异性分别为98.0%和98.4%。确证性阻断酶联免疫吸附试验、临床病史及随访表明该检测的真实特异性为100%。