用于临床标本中单纯疱疹病毒抗原直接检测的自动化免疫诊断分析系统的评估。
Evaluation of an automated immunodiagnostic assay system for direct detection of herpes simplex virus antigen in clinical specimens.
作者信息
Johnston S L, Hamilton S, Bindra R, Hursh D A, Gleaves C A
机构信息
Bellin Memorial Hospital, Green Bay, Wisconsin 54305.
出版信息
J Clin Microbiol. 1992 Apr;30(4):1042-4. doi: 10.1128/jcm.30.4.1042-1044.1992.
The Vitek ImmunoDiagnostic Assay System (VIDAS) is a 2 1/3-h automated qualitative enzyme-linked fluorescent immunoassay developed for the direct detection of herpes simplex virus (HSV) antigen in clinical specimens. A total of 356 clinical specimens submitted for HSV isolation were prospectively evaluated with the VIDAS, and the results of the technique were compared with those of both HSV isolation in cell culture and Herpchek, a nonautomated enzyme immunoassay. Compared to cell culture, VIDAS had a sensitivity of 91.6% and a specificity of 89.3%, with positive and negative predictive values of 82.6 and 95.0%, respectively. In comparison to Herpchek, VIDAS had a sensitivity of 93.7% and a specificity of 93.0%, with positive and negative predictive values of 89.4 and 95.9%, respectively. The results demonstrated that the VIDAS required minimal manipulation in order to produce results comparable to those of Herpchek and HSV isolation in cell culture.
Vitek免疫诊断分析系统（VIDAS）是一种耗时2又1/3小时的自动化定性酶联荧光免疫分析方法，用于直接检测临床标本中的单纯疱疹病毒（HSV）抗原。对总共356份提交用于HSV分离的临床标本进行了前瞻性的VIDAS评估，并将该技术的结果与细胞培养中HSV分离以及非自动化酶免疫分析Herpchek的结果进行了比较。与细胞培养相比，VIDAS的灵敏度为91.6%，特异性为89.3%，阳性预测值和阴性预测值分别为82.6%和95.0%。与Herpchek相比，VIDAS的灵敏度为93.7%，特异性为93.0%，阳性预测值和阴性预测值分别为89.4%和95.9%。结果表明，VIDAS只需极少的操作就能产生与Herpchek以及细胞培养中HSV分离相当的结果。
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