时间门控实现更锐利的低功率 STED 纳米显微镜。
Sharper low-power STED nanoscopy by time gating.
机构信息
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany.
出版信息
Nat Methods. 2011 Jun 5;8(7):571-3. doi: 10.1038/nmeth.1624.
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.
应用脉冲激发并结合时间门控检测可提高连续波受激发射损耗（CW-STED）显微镜中的荧光关-开对比度，从而在使用中等光强的情况下在固定和活细胞中揭示更精细的细节。该方法还可实现使用 CW-STED 光束的超分辨率荧光相关光谱学，如通过量化活细胞的质膜中标记脂质分子的动力学来证明。
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