Han Kyu Young, Willig Katrin I, Rittweger Eva, Jelezko Fedor, Eggeling Christian, Hell Stefan W
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Nano Lett. 2009 Sep;9(9):3323-9. doi: 10.1021/nl901597v.
Charged nitrogen-vacancy (NV) color centers in diamond are excellent luminescence sources for far-field fluorescence nanoscopy by stimulated emission depletion (STED). Here we show that these photostable color centers can be visualized by STED using simple continuous-wave or high repetition pulsed lasers (76 MHz) at wavelengths >700 nm for STED. Furthermore, we show that NV centers can be imaged in three dimensions (3D) inside the diamond crystal and present single-photon signatures of single color centers recorded in high density samples, demonstrating a new recording scheme for STED and related far-field nanoscopy approaches. Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences.
金刚石中的带电氮空位（NV）色心是用于受激发射损耗（STED）远场荧光纳米显微镜的优秀发光源。在此我们表明，这些光稳定色心可通过STED使用简单的连续波或高重复频率脉冲激光器（76 MHz）在波长>700 nm的条件下进行可视化。此外，我们表明NV中心可在金刚石晶体内进行三维（3D）成像，并呈现高密度样品中记录的单个色心的单光子特征，展示了一种用于STED及相关远场纳米显微镜方法的新记录方案。最后，我们举例说明了在STED显微镜中使用含NV中心的纳米金刚石作为发光标签的潜力。我们的结果为纳米光学、纳米技术和生命科学提供了新的实验途径。
