School of Applied Bioscience and Food Science and Biotechology and BK21 Research Team for Developing Functional Health Food Materials, Kyungpook National University, Deagu 702-701, Republic of Korea.
Inflamm Res. 2011 Oct;60(10):909-17. doi: 10.1007/s00011-011-0351-4. Epub 2011 Jun 14.
Given the preventive effect of soy intake against several chronic diseases, this study was conducted to investigate the inhibitory activity against inflammatory response of phytoalexins glyceollins derived from soybean isoflavones by treatment with a biotic elicitor.
Using RAW264.7 cells, we examined the effects of glyceollins on production of nitric oxide (NO) and inflammatory cytokines, expression of inducible nitric oxide synthase (iNOS) and cyclo-oxygenase (COX)-2, and activation of NF-кB, induced by lipopolysaccharide (LPS).
Our data showed that glyceollins effectively inhibited NO production, IL-6 release, and expression of iNOS and COX-2 induced by LPS. In particular, glyceollins suppressed the LPS-induced phosphorylation of NF-кB p65, suggesting that the compounds inhibit the production of NO and transcriptional activation of COX-2 by regulating NF-кB activity. In another experiment we found that glyceollins enhanced the expression of heme oxygenase 1 in LPS-treated RAW264.7 cells. Glyceollins also reduced TPA-induced skin inflammation in a mouse model, confirming the anti-inflammatory activity of glyceollins in an in-vivo system as well as in a cell culture system.
Glyceollins exert an anti-inflammatory effect, which is mediated through the inhibition of NF-κB activation in LPS-activated murine RAW264.7 cells. Glyceollins merit further study as potential therapeutic agents for inflammatory disorders.
鉴于大豆摄入量对多种慢性疾病的预防作用，本研究旨在探讨生物诱导剂处理后大豆异黄酮中的植物抗毒素黄豆苷元对炎症反应的抑制活性。
我们使用 RAW264.7 细胞，研究了黄豆苷元对脂多糖（LPS）诱导的一氧化氮（NO）和炎性细胞因子产生、诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2表达以及 NF-κB 激活的影响。
我们的数据表明，黄豆苷元能有效抑制 LPS 诱导的 NO 产生、IL-6 释放以及 iNOS 和 COX-2 的表达。特别是，黄豆苷元抑制了 LPS 诱导的 NF-κB p65 磷酸化，表明这些化合物通过调节 NF-κB 活性来抑制 NO 的产生和 COX-2 的转录激活。在另一项实验中，我们发现黄豆苷元增强了 LPS 处理的 RAW264.7 细胞中血红素加氧酶 1 的表达。黄豆苷元还减少了 TPA 诱导的小鼠模型中的皮肤炎症，证实了黄豆苷元在体内和细胞培养系统中具有抗炎活性。
黄豆苷元通过抑制 LPS 激活的鼠 RAW264.7 细胞中 NF-κB 的激活发挥抗炎作用。黄豆苷元作为炎症性疾病的潜在治疗剂值得进一步研究。
