Neal J D, Tobian A A R, Laeyendecker O, Ngo T D, Redd A D, Reynolds S J, Ashley Morrow R, Manucci J L, Serwadda D, Gray R H, Quinn T C
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Int J STD AIDS. 2011 Jun;22(6):342-4. doi: 10.1258/ijsa.2009.009327.
Herpes simplex virus type 2 (HSV-2) infection is one of the most common sexually transmitted infections (STIs) worldwide. While glycoprotein G-2 enzyme-linked immunosorbent assays are commonly used for the serological detection of HSV-2 antibodies, they have low specificity in developing countries. The Euroline Western blot (WB) is a commercially available assay that is easy to perform; however, little is known about its performance characteristics. This study evaluated Euroline WB for the detection of HSV-2 antibodies compared with University of Washington Western blot in three geographically different regions: Baltimore, MD, USA; Rakai, Uganda; and Kunming, China. Among the 135 American men attending a STI clinic in Baltimore, MD, 72% (n = 97) were HSV-2-positive by Euroline WB, showing a sensitivity of 97.8% and a specificity of 81.8%. Among the 273 commercial sex workers in Kunming, 62.3% were HSV-2-positive by Euroline WB (sensitivity 96.9%, specificity 89.1%). Among the 437 Ugandans in Rakai, 67.3% were HSV-2-positive by Euroline WB (sensitivity 98.7%, specificity 65.4%). The Euroline WB has a consistently high sensitivity, but specificity varied significantly among the different locations.
2型单纯疱疹病毒（HSV-2）感染是全球最常见的性传播感染之一。虽然糖蛋白G-2酶联免疫吸附测定常用于HSV-2抗体的血清学检测，但在发展中国家其特异性较低。Euroline免疫印迹法是一种易于操作的商用检测方法；然而，对其性能特征了解甚少。本研究在三个地理位置不同的地区——美国马里兰州巴尔的摩、乌干达拉克伊和中国昆明，将Euroline免疫印迹法与华盛顿大学免疫印迹法进行比较，以评估其对HSV-2抗体的检测能力。在马里兰州巴尔的摩一家性传播感染诊所就诊的135名美国男性中，Euroline免疫印迹法检测出HSV-2阳性的比例为72%（n = 97），敏感性为97.8%，特异性为81.8%。在昆明的273名商业性工作者中，Euroline免疫印迹法检测出HSV-2阳性的比例为62.3%（敏感性96.9%，特异性89.1%）。在拉克伊的437名乌干达人中，Euroline免疫印迹法检测出HSV-2阳性的比例为67.3%（敏感性98.7%，特异性65.4%）。Euroline免疫印迹法的敏感性始终很高，但不同地点的特异性差异显著。
