Lingappa J, Nakku-Joloba E, Magaret A, Friedrich D, Dragavon J, Kambugu F, Joloba M, Whalen C, Coombs R, Celum C, Morrow R Ashley
Department of Global Health, University of Washington, Seattle, WA 98102, USA.
Int J STD AIDS. 2010 Sep;21(9):611-6. doi: 10.1258/ijsa.2009.008477.
Herpes simplex virus type 2 (HSV-2) is a risk factor for HIV-1 infection. We characterized HSV-2 serology assay performance in HIV-positive and HIV-negative Africans. Serostatus for HSV-2 and HIV-1 was determined in 493 serum specimens stored from a community HSV-2 prevalence survey in Kampala, Uganda. HSV-2 serology by Focus HerpeSelect ELISA, Biokit HSV-2 rapid assay and Kalon HSV-2 was compared with HSV-2 Western blot (WB) according to HIV-1 serostatus. Sensitivity/specificity was: 99.5%/70.2% for Focus, 97.0%/86.4% for Biokit and 97.5%/96.2% for Kalon. Focus with Biokit confirmation improved sensitivity/specificity (99.4%/96.8%, respectively). Use of a higher Focus index value cut-off of 2.2 instead of 1.1 increased specificity from 70.2% to 92.4%. Kalon had higher specificity than Focus (P < 0.001). Of commercially available HSV-2 serological assays, Kalon alone, or Focus ELISA followed by Biokit confirmation perform best. Improved HSV-2 assays are needed for HSV-2 and HIV-1 public health activities in Africa.
2型单纯疱疹病毒（HSV-2）是HIV-1感染的一个风险因素。我们对HIV阳性和HIV阴性非洲人的HSV-2血清学检测性能进行了特征分析。在乌干达坎帕拉的一项社区HSV-2患病率调查中储存的493份血清标本中确定了HSV-2和HIV-1的血清状态。根据HIV-1血清状态，将Focus HerpeSelect ELISA、Biokit HSV-2快速检测法和Kalon HSV-2的HSV-2血清学检测结果与HSV-2免疫印迹法（WB）进行比较。灵敏度/特异性分别为：Focus检测法为99.5%/70.2%，Biokit检测法为97.0%/86.4%，Kalon检测法为97.5%/96.2%。Focus检测法经Biokit确证后灵敏度/特异性提高（分别为99.4%/96.8%）。将Focus指数值的较高截断值从1.1提高到2.2可使特异性从70.2%提高到92.4%。Kalon检测法的特异性高于Focus检测法（P<0.001）。在市售的HSV-2血清学检测方法中，单独使用Kalon检测法，或先采用Focus ELISA检测法再经Biokit确证的检测方法性能最佳。非洲开展HSV-2和HIV-1公共卫生活动需要改进的HSV-2检测方法。
