Natural Products Laboratory, Department of Chemistry, The University of Burdwan, India.
Int J Biol Macromol. 2011 Nov 1;49(4):637-42. doi: 10.1016/j.ijbiomac.2011.06.024. Epub 2011 Jun 30.
The water-extracted carbohydrate polymers (WE) of Phyllanthus emblica are analyzed using chemical, chromatographic, and spectroscopic methods. Anion-exchange-chromatography of WE yielded four fractions (F1-F4) with different chemical compositions and all of them contain phenolics. The major fraction F4 possesses 50% polysaccharide and 26% phenol, and is a glycoconjugate. The antioxidant capacities of WE and F4 are comparable to standard anti-oxidants. Notably, activities of F1-F4 correlate with their phenol content. Evidence for the complexation of F4 with bovine serum albumin is presented by fluorescence quenching measurement. The results also indicate conformational change of protein at high carbohydrate polymer concentration.
采用化学、色谱和光谱方法分析余甘子水提碳水化合物聚合物（WE）。WE 的阴离子交换色谱分离得到四个组分（F1-F4），它们具有不同的化学成分，且均含有酚类物质。主要组分 F4 含有 50%多糖和 26%酚类物质，是一种糖缀合物。WE 和 F4 的抗氧化能力与标准抗氧化剂相当。值得注意的是，F1-F4 的活性与其酚含量相关。荧光猝灭测量结果表明 F4 与牛血清白蛋白的络合作用。研究结果还表明，在高碳水化合物聚合物浓度下蛋白质构象发生变化。
