Cassidy L F, Lyles D S, Abramson J S
Department of Microbiology and Immunology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
J Clin Microbiol. 1988 Jul;26(7):1267-70. doi: 10.1128/jcm.26.7.1267-1270.1988.
Various reports have indicated that infection of polymorphonuclear leukocytes (PMNL) with influenza virus causes depression of their metabolic and chemotactic responses, but the effect the PMNL has on the life cycle of influenza virus has not been well defined. The studies reported here were undertaken to determine whether influenza virus could replicate within PMNL. Virus-infected and uninfected PMNL were labeled with [35S]methionine and analyzed by gel electrophoresis and fluorography for detection of newly synthesized proteins. Both host- and virus-specific proteins were produced within PMNL. By using indirect immunofluorescence techniques combined with flow cytometry, the expression of newly synthesized viral antigens was detected in virus-infected PMNL. Plaque assays on supernatant fluid from infected PMNL showed that infectious progeny were not produced, indicating that influenza virus infection of PMNL is abortive.
各种报告表明，流感病毒感染多形核白细胞（PMNL）会导致其代谢和趋化反应受到抑制，但PMNL对流感病毒生命周期的影响尚未明确界定。本文所报道的研究旨在确定流感病毒是否能在PMNL内复制。用[35S]甲硫氨酸标记病毒感染和未感染的PMNL，并通过凝胶电泳和荧光自显影分析来检测新合成的蛋白质。在PMNL内产生了宿主特异性和病毒特异性蛋白质。通过将间接免疫荧光技术与流式细胞术相结合，在病毒感染的PMNL中检测到了新合成病毒抗原的表达。对感染PMNL的上清液进行噬斑测定表明未产生有感染性的子代，这表明PMNL的流感病毒感染是流产性的。
