School of Public Health, Seoul National University, 599 Gwanak-ro, Gwanak, Seoul 151-742, South Korea.
Environ Monit Assess. 2012 Jun;184(6):3687-95. doi: 10.1007/s10661-011-2216-2. Epub 2011 Jul 16.
Freshwater cladocera such as Daphnia magna and Ceriodaphnia dubia have been used extensively for freshwater toxicity test worldwide. However, these species may not be indigenous in certain geographical regions, which restrict the utility of these organisms as test species. In the present study, we investigated optimal culture and test conditions for an indigenous freshwater macroinvertebrate of Korea, Moina macrocopa. The culture conditions that were evaluated included water temperature (20°C and 25°C), rearing media (moderately hard water or MHW, with or without selenium supplementation, or Elendt M4), and food density (2.5 × 10(7) and 5 × 10(7) cells/mL of Selenastrum capricornutum), and their effects on the life history characteristics of M. macrocopa were determined. Population growth rate of M. macrocopa was maximized at 25°C with 5 × 10(7) cells/mL of algal food density in MHW. A series of chronic three brood reference toxicant tests were conducted under the ideal culture conditions that were identified here, and the results of the tests indicated reliable reproducibility of the test protocol. Optimal culture and test conditions that were identified for M. macrocopa in the present study are suggested for evaluation of chronic toxicity of chemicals and industrial or municipal discharge.
淡水枝角类动物，如大型溞（Daphnia magna）和双毛额溞（Ceriodaphnia dubia），已被广泛用于全球淡水毒性测试。然而，在某些地理区域，这些物种可能不是本地物种，这限制了这些生物作为测试物种的用途。在本研究中，我们研究了韩国本土淡水大型底栖无脊椎动物——霍甫水蚤（Moina macrocopa）的最佳培养和测试条件。评估的培养条件包括水温（20°C 和 25°C）、养殖介质（中硬度水或 MHW，是否添加硒）和食物密度（2.5×10^7 和 5×10^7 个细胞/mL 的衣藻），并确定了这些条件对 M. macrocopa 生活史特征的影响。在 MHW 中，温度为 25°C，藻类食物密度为 5×10^7 个细胞/mL 时，M. macrocopa 的种群增长率达到最大值。在确定的理想培养条件下，进行了一系列慢性三代参考毒物测试，测试结果表明测试方案具有可靠的重现性。本研究确定的 M. macrocopa 的最佳培养和测试条件，建议用于评估化学品和工业或市政排放物的慢性毒性。
