无标记键选择性振动激发分子成像。
Label-free bond-selective imaging by listening to vibrationally excited molecules.
机构信息
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Phys Rev Lett. 2011 Jun 10;106(23):238106. doi: 10.1103/PhysRevLett.106.238106.
Abstract
We report the realization of vibrational photoacoustic (VPA) microscopy using optical excitation of molecular overtone vibration and acoustic detection of the resultant pressure transients. Our approach eliminates the tissue scattering problem encountered in near-infrared spectroscopy and enables depth-resolved signal collection. The 2nd overtone of the CH bond stretch around 8300 cm(-1), where blood interference is minimal, is excited. We demonstrate 3D VPA imaging of lipid-rich atherosclerotic plaques by excitation from the artery lumen, and lipid storage in live Drosophila larvae, with millimeter scale penetration depth [corrected].
摘要
我们报告了使用分子倍频振动的光学激发和对所得压力瞬变的声学检测来实现振动光声（VPA）显微镜。我们的方法消除了近红外光谱中遇到的组织散射问题，并实现了深度分辨的信号采集。在血液干扰最小的 8300 cm(-1) 左右，激发 CH 键伸缩的第二泛频。我们通过从动脉管腔激发展示了富含脂质的动脉粥样硬化斑块的 3D VPA 成像，以及活果蝇幼虫中的脂质储存，具有毫米级的穿透深度[已更正]。
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