Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de, 3029, Sfax, Tunisia.
Biol Proced Online. 2011 Jul 21;13(1):5. doi: 10.1186/1480-9222-13-5.
DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.
由于新型隐球菌有一层厚厚的耐药荚膜,因此从该菌中提取 DNA 较为困难。我们优化了一种新型、快速的提取新型隐球菌 DNA 的方法,即采用短时间尿素处理,随后用快速 Chelex 树脂沉淀法。该方法比以前报道的方法更简单。