Cardoso Tereza Cristina, Antello Talita Fontes, Vieira Flávia Volpato, Machado Saulo Tarso Zacarias, Nogueira Adriana Hellmeister de Campos
Laboratory of Animal Virology, Faculty of Veterinary Medicine, Rua Clóvis Pestana, 793, 16.050-680, Araçatuba, São Paulo, Brazil.
Trop Anim Health Prod. 2012 Mar;44(3):379-82. doi: 10.1007/s11250-011-9929-7. Epub 2011 Jul 24.
Brazil represents the greatest beef producer among tropical countries, and the major obstacle for meat international trade is sanitary problems especially closely related to viral encephalitis. The goal of this study was to estimate the accuracy of the glycol and US9 gene-based polymerase chain reactions (PCRs) for the detection of bovine Herpesvirus type 5 (BoHV-5) from decomposed brain samples (n = 95). For this purpose, a latent-class (bayesian) approach was used. Sensitivity (Se) was estimated to be 70% (95% probability interval, 40-80) and specificity (Sp) 100% in the statistical analysis for both PCRs used. Accordingly, a minimum of ≥40% of the calves was estimated to harbor BoHV-5 DNA even after 72 h of decomposition at room temperature. It was concluded that US9 gene-based PCR could also be considered a cost-effective alternative in sanitary programmers. However, given the importance of veterinary diagnoses, PCR-positive samples should be further confirmed through in vitro isolation and/or sequencing.
巴西是热带国家中最大的牛肉生产国，肉类国际贸易的主要障碍是卫生问题，尤其是与病毒性脑炎密切相关的问题。本研究的目的是评估基于乙二醇和US9基因的聚合酶链反应（PCR）从分解的脑样本（n = 95）中检测牛疱疹病毒5型（BoHV-5）的准确性。为此，采用了潜在类别（贝叶斯）方法。在对所使用的两种PCR的统计分析中，灵敏度（Se）估计为70%（95%概率区间，40 - 80），特异性（Sp）为100%。因此，估计至少40%的小牛即使在室温下分解72小时后仍携带BoHV-5 DNA。得出的结论是，基于US9基因的PCR在卫生程序中也可被视为一种具有成本效益的替代方法。然而，鉴于兽医诊断的重要性，PCR阳性样本应通过体外分离和/或测序进一步确认。
