Multiple Sclerosis Center, Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China.
J Clin Neurosci. 2011 Oct;18(10):1313-7. doi: 10.1016/j.jocn.2011.01.031. Epub 2011 Jul 26.
Growing evidence suggests that interleukin (IL)-17 and IL-17-secreting CD4(+)T (Th17) cells are involved in the pathogenic mechanisms of multiple sclerosis (MS). IL-17-secreting CD8(+)T cells were recently identified as a novel subset of CD8(+)T cells. We aimed to analyze the role of Th17 and IL-17 secreting CD8(+)T cells in the pathogenesis of neuromyelitis optica (NMO) as well as MS. Fourteen patients with NMO, 20 with MS and 16 control participants (CTL) were enrolled between November 2008 and December 2009. The proportion of Th17 cells and IL-17 secreting CD8(+)T cells were counted using flow cytometry, and serum levels of IL-6, IL-17, IL-21, IL-23, and transforming growth factor-beta (TGF-β) were measured by enzyme-linked immunosorbent assay. Patients with NMO had a larger proportion of Th17 cells than patients with MS (3.72% versus [vs.] 2.58%, p=0.02) and CTL (3.72% vs. 1.36%, p<0.001). The proportion of Th17 cells in patients with MS was also markedly higher than in the CTL (2.58% vs. 1.36%, p<0.001). IL-17-secreting CD8(+)T cell counts in NMO patients were markedly higher than in MS patients (1.61% vs. 1.09%, p=0.036) and CTLs (1.61% vs. 0.58%, p<0.001). The proportion of IL-17-secreting CD8(+)T cells in MS patients was also higher than in CTLs (1.09% vs. 0.58%, p=0.002). Serum IL-17 and IL-23 levels were increased in patients with NMO and MS, while serum IL-21 concentration was higher only in NMO patients compared to CTL. We concluded that Th17 cells were highly activated in patients with NMO. IL-17-secreting CD8(+)T cells were increased in patients with NMO and MS during relapse and have an important role in the pathological mechanism of NMO and MS.
越来越多的证据表明白细胞介素（IL）-17 和分泌白细胞介素-17 的 CD4+T（Th17）细胞参与多发性硬化症（MS）的发病机制。最近发现白细胞介素-17 分泌的 CD8+T 细胞是 CD8+T 细胞的一个新亚群。我们旨在分析 Th17 和分泌白细胞介素-17 的 CD8+T 细胞在视神经脊髓炎（NMO）和 MS 发病机制中的作用。2008 年 11 月至 2009 年 12 月期间，共纳入 14 例 NMO 患者、20 例 MS 患者和 16 名对照参与者（CTL）。采用流式细胞术计数 Th17 细胞和分泌白细胞介素-17 的 CD8+T 细胞的比例，采用酶联免疫吸附试验检测血清白细胞介素-6、白细胞介素-17、白细胞介素-21、白细胞介素-23 和转化生长因子-β（TGF-β）水平。NMO 患者的 Th17 细胞比例明显高于 MS 患者（3.72%比[与]2.58%，p=0.02）和 CTL（3.72%比 1.36%，p<0.001）。MS 患者的 Th17 细胞比例也明显高于 CTL（2.58%比 1.36%，p<0.001）。NMO 患者的分泌白细胞介素-17 的 CD8+T 细胞计数明显高于 MS 患者（1.61%比 1.09%，p=0.036）和 CTL（1.61%比 0.58%，p<0.001）。MS 患者的分泌白细胞介素-17 的 CD8+T 细胞比例也高于 CTL（1.09%比 0.58%，p=0.002）。NMO 和 MS 患者的血清白细胞介素-17 和白细胞介素-23 水平升高，而 NMO 患者的血清白细胞介素-21 浓度仅高于 CTL。我们得出结论，NMO 患者的 Th17 细胞高度激活。NMO 和 MS 患者复发时，分泌白细胞介素-17 的 CD8+T 细胞增加，在 NMO 和 MS 的病理机制中具有重要作用。
