Grenoble Electrical Engineering Laboratory (G2Elab), UMR 5269 (Grenoble-INP, UJF, CNRS), BP 46, 38402, Saint Martin d'Hères Cedex, France.
Lab Chip. 2011 Sep 21;11(18):3153-61. doi: 10.1039/c1lc20232d. Epub 2011 Aug 1.
Cell arrays are of foremost importance for many applications in pharmaceutical research or fundamental biology. Although arraying techniques have been widely investigated for adherent cells, organization of cells in suspension has been rarely considered. The arraying of non-adherent cells using the diamagnetic repulsive force is presented. A planar arrangement of Jurkat cells is achieved at the microscale above high quality microfabricated permanent magnets with remanent magnetization of J(r)≈ 1 T, in the presence of a paramagnetic contrast agent. The cytotoxicity of three Gd based contrast agents, Gd-DOTA, Gd-BOPTA and Gd-HP-DO3A, is studied. Among them, Gd-HP-DO3A appears to be the most biocompatible toward Jurkat cells. In close agreement with analytical simulations, diamagnetically 'suspended' cells have been successfully arrayed above square and honeycomb-like micromagnet arrays, which act as a "diamagnetophobic" surface. Living cell trapping is achieved in a simple manner using concentrations of Gd-HP-DO3A as low as 1.5 mM.
细胞阵列对于药物研究或基础生物学的许多应用都至关重要。尽管已经广泛研究了用于贴壁细胞的排列技术，但对悬浮细胞的组织排列却很少考虑。本文提出了利用抗磁性斥力对非贴壁细胞进行排列的方法。在存在顺磁性对比剂的情况下，在具有剩余磁化强度 J(r)≈1 T 的高质量微制造永磁体上方的微尺度上，实现了 Jurkat 细胞的平面排列。研究了三种基于 Gd 的对比剂 Gd-DOTA、Gd-BOPTA 和 Gd-HP-DO3A 的细胞毒性。其中，Gd-HP-DO3A 对 Jurkat 细胞表现出最高的生物相容性。与分析模拟非常吻合的是，成功地在正方形和蜂窝状微磁体阵列上方排列了抗磁性“悬浮”细胞，这些阵列充当了“抗磁斥性”表面。使用低至 1.5 mM 的 Gd-HP-DO3A 浓度即可简单地实现活细胞捕获。
