Department of Bioengineering, Izmir Institute of Technology, Urla, Izmir, Turkey.
Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, Turkey.
Sci Rep. 2018 May 8;8(1):7239. doi: 10.1038/s41598-018-25718-9.
Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material and biological sciences. Yet little is known about the ability of the magnetic levitation system to facilitate biofabrication of in situ three dimensional (3D) cellular structures. Here, we optimized a magnetic levitation though negative magnetophoresis protocol appropriate for long term levitated cell culture and developed an in situ 3D cellular assembly model with controlled cluster size and cellular pattern under simulated weightlessness. The developed strategy outlines a potential basis for the study of weightlessness on 3D living structures and with the opportunity for real-time imaging that is not possible with current ground-based simulated weightlessness techniques. The low-cost technique presented here may offer a wide range of biomedical applications in several research fields, including mechanobiology, drug discovery and developmental biology.
磁悬浮通过负磁移动是一种模拟失重的新技术，最近在材料和生物科学领域得到了应用。然而，对于磁悬浮系统促进原位三维（3D）细胞结构生物制造的能力知之甚少。在这里，我们优化了一种适用于长期悬浮细胞培养的磁悬浮通过负磁移动方案，并在模拟失重条件下开发了一种具有受控簇大小和细胞模式的原位 3D 细胞组装模型。所开发的策略为研究 3D 活体结构上的失重提供了潜在的基础，并提供了实时成像的机会，这是当前基于地面的模拟失重技术所不可能实现的。这里提出的低成本技术可能在包括机械生物学、药物发现和发育生物学在内的多个研究领域提供广泛的生物医学应用。
