Department of Mechanical and Process Engineering, Particle Technology Laboratory, Institute of Process Engineering, Swiss Federal Institute of Technology Zurich (ETH Zurich), Zurich, Switzerland.
Nanotoxicology. 2012 Sep;6(6):680-90. doi: 10.3109/17435390.2011.604439. Epub 2011 Aug 2.
A novel method is presented which is suitable for assessing in vivo the link between the physicochemical properties of engineered nanomaterials (ENM) and their biological outcomes. The ability of the technique to generate a variety of industry-relevant, property-controlled ENM exposure atmospheres for inhalation studies was systematically investigated. The primary particle size for Fe(2)O(3), SiO(2), Ag and Ag/SiO(2) was controlled from 4 to 25 nm, while the corresponding agglomerate mobility diameter of the aerosol was also controlled and varied from 40 to 120 nm. The suitability of the technique to characterize the pulmonary and cardiovascular effects of inhaled ENMs in intact animal models is also demonstrated using in vivo chemiluminescence (IVCL). The IVCL technique is a highly sensitive method for identifying cardiopulmonary responses to inhaled ENMs under relatively small doses and acute exposures. It is shown that moderate and acute exposures to inhaled nanostructured Fe(2)O(3) can cause both pulmonary and cardiovascular effects.
提出了一种新方法，适用于评估工程纳米材料（ENM）的物理化学性质与其生物结果之间的联系。系统研究了该技术产生各种与工业相关的、受控制的 ENM 暴露气氛以进行吸入研究的能力。Fe(2)O(3)、SiO(2)、Ag 和 Ag/SiO(2)的初级粒径从 4 到 25nm 进行控制，而气溶胶的相应团聚体迁移直径也从 40 到 120nm 进行控制和变化。还使用体内化学发光（IVCL）证明了该技术在完整动物模型中表征吸入 ENM 的肺和心血管效应的适用性。IVCL 技术是一种高度灵敏的方法，可在相对较小的剂量和急性暴露下识别吸入 ENM 的心肺反应。结果表明，适度和急性暴露于吸入的纳米结构 Fe(2)O(3)可引起肺部和心血管效应。
