从纹带棒状杆菌诱导的一种新型温和噬菌体的全基因组分析。
Complete genome analysis of a novel temperate bacteriophage induced from Corynebacterium striatum.
机构信息
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, Shandong, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
出版信息
Arch Virol. 2019 Nov;164(11):2877-2880. doi: 10.1007/s00705-019-04370-2. Epub 2019 Aug 27.
A temperate bacteriophage, IME1320_01, was induced by mitomycin C treatment from Corynebacterium striatum. This phage possesses a double-stranded DNA genome of 40,086 bp with a G+C content of 58%. A total of 53 putative open reading frames (ORFs) were identified in its genome. BLASTn analysis revealed that IME1320_01 had the highest sequence similarity to Corynebacterium striatum strain 216, with a genome homology coverage of 44% and highest sequence identity of 95%. The termini of the phage genome was non-redundant, with a 13-nt 3'-protruding cohesive end. To the best of our knowledge, phage IME1320_01 is the first inducible phage to be identified in Corynebacterium striatum.
一株温和噬菌体 IME1320_01 经丝裂霉素 C 诱导自棒状杆菌属的条纹状亚种分离而得。该噬菌体拥有一个 40086bp 的双链 DNA 基因组，GC 含量为 58%。在其基因组中总共鉴定出 53 个推定的开放阅读框（ORFs）。BLASTn 分析表明，IME1320_01 与棒状杆菌属菌株 216 具有最高的序列相似性，基因组同源性覆盖率为 44%，最高序列同一性为 95%。噬菌体基因组的末端是非冗余的，具有 13 个核苷酸的 3’突出粘性末端。据我们所知，噬菌体 IME1320_01 是首次在棒状杆菌属条纹状亚种中鉴定到的可诱导噬菌体。
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